Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China.
College of Science, Hunan Agricultural University, Changsha 410128, China.
Talanta. 2018 Aug 15;186:473-480. doi: 10.1016/j.talanta.2018.04.060. Epub 2018 Apr 22.
In this study, an intelligent and label-free sensor is utilized for the first time to one-spot simultaneous detection hepatocellular carcinoma markers AFP and miRNA-122 by a resonance light scattering (RLS) sensor. cDNA1 hybridizes with cDNA2 to form double-stranded DNA (dsDNA). The construction of dsDNA and methyl violet is used to form the RLS sensor via the electronic interaction. When AFP or miRNA-122 is present, the cDNA (cDNA1 or cDNA2) can bindings of target, thereby RLS intensity changed proportionally with the concentration of AFP or that of miRNA-122. The detection limits of AFP and miRNA-122 are 0.94 μg/L and 98 pM respectively, and their good linear which ranges from 5 to 100 μg/L and 200 pM to 10 nM are achieved using the assay. In the presence of miRNA-122 and AFP mixtures, AFP bound to the AFP aptamer to increase the RLS signal, and miRNA-122 bound to the miRNA-122 complementary strand to decrease the RLS signal. The RLS signal changed in response to changing AFP and miRNA-122 concentrations, so that one-spot simultaneous detection of alpha fetal protein and miRNA-122 is achieved. This method has potential practical applications in the research of hepatocellular carcinoma.
在这项研究中，首次利用智能无标记传感器通过共振光散射（RLS）传感器进行单点同时检测肝癌标志物 AFP 和 miRNA-122。cDNA1 与 cDNA2 杂交形成双链 DNA（dsDNA）。dsDNA 与甲基紫的构建用于通过电子相互作用形成 RLS 传感器。当存在 AFP 或 miRNA-122 时，cDNA（cDNA1 或 cDNA2）可以与靶标结合，从而 RLS 强度与 AFP 或 miRNA-122 的浓度成正比变化。AFP 和 miRNA-122 的检测限分别为 0.94μg/L 和 98pM，使用该测定法可实现 5 至 100μg/L 和 200pM 至 10nM 的良好线性范围。在 miRNA-122 和 AFP 混合物存在的情况下，AFP 与 AFP 适体结合以增加 RLS 信号，而 miRNA-122 与 miRNA-122 互补链结合以降低 RLS 信号。RLS 信号响应 AFP 和 miRNA-122 浓度的变化而变化，从而实现对 AFP 和 miRNA-122 的单点同时检测。该方法在肝癌研究中具有潜在的实际应用。
