Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, Anhui 230022, China.
Department of Endocriology, Anhui Medical University, Hefei, Anhui 230032, China.
Biosci Rep. 2018 Jul 2;38(4). doi: 10.1042/BSR20171731. Print 2018 Aug 31.
The present study aimed to investigate the effect of maternal hypothyroidism during pregnancy on thyroid function of the fetal rat. Female Sprague-Dawley rats were randomized into two groups. Propylthiouracil (PTU) group received PTU in drinking water for 6 weeks (=90), normal group received normal drinking water (=50). The pregnant rats were obtained and had a cesarean-section to get at gestational ages of 8.5, 13, and 21 days, following blood samples and skeletal muscle were obtained from fetal rats. Levels of thyroid hormone, insulin, mitochondrial protein, and adipokines were detected using ELISA. Western blotting was performed to analyze mitochondria and insulin signal transduction-related protein in fetal rat skeletal muscle. Immunostaining of Periodic Acid-Schiff (PAS) and Oil Red O was used to observe the accumulation of muscle glycogen and lipid in the fetal rat. The results showed that the levels of thyroid hormone, insulin, insulin signal transduction-related protein, mitochondrial, and adipokines increased with the fetus developed, but had no statistical differences in the PTU group compared with the normal group. In conclusion, pregnant rats with hypothyroidism had no influence on insulin resistance (IR), lipid accumulation, and mitochondrial dysfunction in skeletal muscle of the fetal rats.
本研究旨在探讨妊娠期间母体甲状腺功能减退对胎鼠甲状腺功能的影响。雌性 Sprague-Dawley 大鼠随机分为两组。PTU 组在饮用水中接受 PTU 治疗 6 周（=90），正常组接受正常饮用水（=50）。获得怀孕大鼠，并进行剖宫产以获得妊娠 8.5、13 和 21 天的胎儿大鼠，随后从胎儿大鼠中获得血液样本和骨骼肌。使用 ELISA 检测甲状腺激素、胰岛素、线粒体蛋白和脂肪细胞因子的水平。通过 Western blot 分析胎儿大鼠骨骼肌中线粒体和胰岛素信号转导相关蛋白。使用过碘酸希夫（PAS）和油红 O 免疫染色观察胎儿大鼠肌肉糖原和脂质的积累。结果表明，随着胎儿的发育，甲状腺激素、胰岛素、胰岛素信号转导相关蛋白、线粒体和脂肪细胞因子的水平增加，但与正常组相比，PTU 组无统计学差异。总之，甲状腺功能减退的孕鼠对胎儿大鼠骨骼肌的胰岛素抵抗（IR）、脂质积累和线粒体功能障碍没有影响。
