Rong Dawei, Dong Chaoxi, Fu Kai, Wang Hanjin, Tang Weiwei, Cao Hongyong
Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
Onco Targets Ther. 2018 May 11;11:2753-2761. doi: 10.2147/OTT.S156516. eCollection 2018.
Circular RNAs (circRNAs), which have closed-loop structure, are involved in the pathogenesis of human diseases including various types of carcinomas. The present study aimed to investigate the relationship between a new circular RNA named circ_0066444 and gastric cancer (GC) carcinogenesis.
The circ_0066444 levels in 106 paired gastric carcinoma tissues and related adjacent normal tissues were detected by real-time quantitative reverse-transcription polymerase chain reaction. The correlation between the expression of circ_0066444 and clinicopathological features was analyzed. The impact of circ_0066444 expression on cell proliferation, invasion, as well as migration was evaluated in vitro using knockdown expression strategies. Finally, a network of circ_0066444-targeted miRNA interactions and their corresponding mRNAs was constructed.
circ_0066444 was found to be significantly upregulated in 106 GC tissues as compared with paired adjacent nontumorous tissues (=0.025), showing a high positive correlation with lymphatic metastasis (=0.023). Furthermore, in vitro assays of the GC cell lines BGC-823 and AGS demonstrated that knockdown of circ_0066444 reduced cell proliferation, invasion, and migration significantly. Prediction and annotation revealed circ_0066444 was able to sponge to 5 miRNAs and 15 corresponding target mRNAs.
Our study indicated upregulation of circ_0066444 promotes gastric cell proliferation, invasion, and migration ability and might serve as a novel biomarker for GC.
具有闭环结构的环状RNA（circRNAs）参与包括各种类型癌症在内的人类疾病的发病机制。本研究旨在探讨一种名为circ_0066444的新型环状RNA与胃癌（GC）发生之间的关系。
采用实时定量逆转录聚合酶链反应检测106对胃癌组织及相关癌旁正常组织中circ_0066444的水平。分析circ_0066444表达与临床病理特征之间的相关性。采用敲低表达策略在体外评估circ_0066444表达对细胞增殖、侵袭和迁移的影响。最后，构建了circ_0066444靶向的miRNA相互作用及其相应mRNA的网络。
与配对的相邻非肿瘤组织相比，在106例GC组织中发现circ_0066444显著上调（=0.025），与淋巴转移呈高度正相关（=0.023）。此外，对GC细胞系BGC-823和AGS的体外分析表明，敲低circ_0066444可显著降低细胞增殖、侵袭和迁移。预测和注释显示circ_0066444能够吸附5种miRNA和15种相应的靶mRNA。
我们的研究表明，circ_0066444的上调促进了胃细胞的增殖、侵袭和迁移能力，可能作为GC的一种新型生物标志物。
