Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Zografou, Athens, Greece.
Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
Brain Behav. 2018 Jun;8(6):e01001. doi: 10.1002/brb3.1001. Epub 2018 May 22.
The widespread use of wireless devices during the last decades is raising concerns about adverse health effects of the radiofrequency electromagnetic radiation (RF-EMR) emitted from these devices. Recent research is focusing on unraveling the underlying mechanisms of RF-EMR and potential cellular targets. The "omics" high-throughput approaches are powerful tools to investigate the global effects of RF-EMR on cellular physiology.
In this work, C57BL/6 adult male mice were whole-body exposed (n = 8) for 2 hr to GSM 1800 MHz mobile phone radiation at an average electric field intensity range of 4.3-17.5 V/m or sham-exposed (n = 8), and the RF-EMR effects on the hippocampal lipidome and transcriptome profiles were assessed 6 hr later.
The data analysis of the phospholipid fatty acid residues revealed that the levels of four fatty acids [16:0, 16:1 (6c + 7c), 18:1 9c, eicosapentaenoic acid omega-3 (EPA, 20:5 ω3)] and the two fatty acid sums of saturated and monounsaturated fatty acids (SFA and MUFA) were significantly altered (p < 0.05) in the exposed group. The observed changes indicate a membrane remodeling response of the tissue phospholipids after nonionizing radiation exposure, reducing SFA and EPA, while increasing MUFA residues. The microarray data analysis demonstrated that the expression of 178 genes changed significantly (p < 0.05) between the two groups, revealing an impact on genes involved in critical biological processes, such as cell cycle, DNA replication and repair, cell death, cell signaling, nervous system development and function, immune system response, lipid metabolism, and carcinogenesis.
This study provides preliminary evidence that mobile phone radiation induces hippocampal lipidome and transcriptome changes that may explain the brain proteome changes and memory deficits previously shown by our group.
过去几十年来，无线设备的广泛使用引起了人们对这些设备发射的射频电磁辐射（RF-EMR）对健康产生不利影响的担忧。最近的研究集中在揭示 RF-EMR 的潜在细胞靶点和潜在的细胞机制。“组学”高通量方法是研究 RF-EMR 对细胞生理学的整体影响的有力工具。
在这项工作中，C57BL/6 成年雄性小鼠全身暴露（n = 8）于 GSM 1800MHz 移动电话辐射 2 小时，平均电场强度范围为 4.3-17.5 V/m 或假暴露（n = 8），然后评估 RF-EMR 对海马脂质组和转录组谱的影响 6 小时后。
对磷脂脂肪酸残基的数据分析表明，四种脂肪酸[16：0、16：1（6c + 7c）、18：1 9c、二十碳五烯酸 omega-3（EPA，20：5 ω3）]和饱和脂肪酸和单不饱和脂肪酸（SFA 和 MUFA）的两个脂肪酸总和的水平在暴露组中显著改变（p < 0.05）。观察到的变化表明组织磷脂在非电离辐射暴露后发生了膜重塑反应，降低了 SFA 和 EPA，同时增加了 MUFA 残基。微阵列数据分析表明，两组之间有 178 个基因的表达发生了显著变化（p < 0.05），这表明对参与关键生物学过程的基因产生了影响，如细胞周期、DNA 复制和修复、细胞死亡、细胞信号转导、神经系统发育和功能、免疫系统反应、脂质代谢和致癌作用。
本研究初步证明，手机辐射诱导海马脂质组和转录组发生变化，这可能解释了我们小组先前显示的大脑蛋白质组变化和记忆缺陷。
