Sygusch J, Allaire M
Département de Biochimie, Faculté de Médecine, Université de Sherbrooke, Fleurimont, Québec, Canada.
Acta Crystallogr A. 1988 Jul 1;44 ( Pt 4):443-8. doi: 10.1107/s0108767388001394.
Radiation damage in protein crystals is described in terms of a sequential process of protein disordering. A new radiation-damage model has been tested against data from several protein crystals and can describe radiation damage corresponding to loss of the original intensity in excess of 80%. The model is an extension of previous models which characterize radiation damage in terms of successive conformational transitions of the protein from an undamaged to a spatially disordered to finally an amorphous state. The proposed model provides a more-general positional characterization of the disordered protein and includes, prior to the disordered state, a new dose-dependent state in which the protein conformation resembles the undamaged protein. Comparison of this model with the best previous model shows that the proposed model provides an improved fit to radiation-damage data.
蛋白质晶体中的辐射损伤是根据蛋白质无序化的连续过程来描述的。一个新的辐射损伤模型已针对来自几种蛋白质晶体的数据进行了测试，并且能够描述与原始强度损失超过80%相对应的辐射损伤。该模型是先前模型的扩展，先前模型根据蛋白质从未受损到空间无序再到最终无定形状态的连续构象转变来表征辐射损伤。所提出的模型提供了对无序蛋白质更一般的位置表征，并且在无序状态之前包括一种新的剂量依赖性状态，其中蛋白质构象类似于未受损的蛋白质。将该模型与之前最好的模型进行比较表明，所提出的模型对辐射损伤数据的拟合度更高。
