Department of Food Analysis, Institute of Agricultural and Food Biotechnology, Rakowiecka, Warsaw, Poland.
Department of Fermentation Technology, Institute of Agricultural and Food Biotechnology, Rakowiecka, Warsaw, Poland.
PLoS One. 2018 May 22;13(5):e0197907. doi: 10.1371/journal.pone.0197907. eCollection 2018.
The aim of this work was to evaluate the activity of xenobiotic mixtures containing persistent organic pollutants isolated from commercial fish oil samples against sex hormone receptors, including estrogen and androgen. The applied bioassay was based on transgenic yeast strains. The mixtures were extracted from the samples using the semi-permeable membrane dialysis technique and analyzed with gas chromatography/ion trap mass spectrometry. It turned out that mixtures of chemicals isolated from fish oil may interact with human steroid sex hormone receptors in various ways: the tested samples showed both estrogenic and anti-androgenic activity. Calculated 17β-estradiol equivalents for the tested samples ranged between 0.003 and 0.073 pg g-1 (fat). Anti-androgenic activity expressed as the flutamide equivalent concentration was in the 18.58-216.21 ng g-1 (fat) range. Polychlorinated biphenyls and various DDT metabolites were the main fish oil pollutants influencing the receptors. Additivity and/or synergy between chemicals was observed in the ER/AR mediated response.
本工作旨在评估从商业鱼油样品中分离出的含有持久性有机污染物的外源化学混合物对包括雌激素和雄激素在内的性激素受体的活性。应用的生物测定基于转基因酵母菌株。使用半透膜透析技术从样品中提取混合物，并使用气相色谱/离子阱质谱进行分析。结果表明，从鱼油中分离出的化学混合物可能以各种方式与人类甾体性激素受体相互作用：测试样品表现出雌激素和抗雄激素活性。测试样品的 17β-雌二醇当量在 0.003 至 0.073 pg g-1（脂肪）之间。以氟他胺当量浓度表示的抗雄激素活性在 18.58 至 216.21 ng g-1（脂肪）范围内。多氯联苯和各种滴滴涕代谢物是影响受体的主要鱼油污染物。在 ER/AR 介导的反应中观察到化学物质的相加和/或协同作用。
