Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
Mikrochim Acta. 2018 May 22;185(6):308. doi: 10.1007/s00604-018-2824-4.
Amorphous titania was modified with boric acid, and the resulting material was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray powder diffraction and X-ray photoelectron spectrometry. The new material, in contrast to conventional boronate affinity materials containing boronic acid ligands, bears boric acid groups. It is shown to exhibit high specificity for glycoproteins, and this was applied to design a method for solid phase extraction of glycoproteins as shown for ribonuclease B, horse radish peroxidase and ovalbumin. Glycoproteins were captured under slightly alkaline environment and released in acidic solutions. The glycoproteins extracted were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The binding capacities for ribonuclease B, horse radish peroxidase and ovalbumin typically are 9.3, 26.0 and 53.0 mg ∙ g, respectively. The method was successfully applied to the selective enrichment of ovalbumin from egg white. Graphical abstract Schematic presentation of the capture of glycoproteins by amorphous titania modified with boric acid.
硼酸改性无定形二氧化钛，并通过扫描电子显微镜、傅里叶变换红外光谱、X 射线粉末衍射和 X 射线光电子能谱对所得材料进行了表征。与含有硼酸配体的传统硼酸亲和材料相比，新材料含有硼酸基团。结果表明，该新材料对糖蛋白具有很高的特异性，并将其应用于糖蛋白的固相萃取方法设计，以核糖核酸酶 B、辣根过氧化物酶和卵清蛋白为例。糖蛋白在弱碱性环境下被捕获，并在酸性溶液中被释放。提取的糖蛋白通过基质辅助激光解吸/电离飞行时间质谱进行检测。核糖核酸酶 B、辣根过氧化物酶和卵清蛋白的结合容量分别为 9.3、26.0 和 53.0mg/g。该方法成功地应用于从蛋清中选择性富集卵清蛋白。
