Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China.
Mikrochim Acta. 2020 Feb 12;187(3):165. doi: 10.1007/s00604-020-4145-7.
For the first time, boronate affinity chromatography and metal oxide affinity chromatography mechanisms for cis-diol-compounds extraction are simultaneously realized on a single material, termed borated zirconia. This material was prepared by hydrolyzing zirconium butoxide with boric acid under non-aqueous environment. The diameter of formed particles is around 200 nm. By extracting catechol under different pH conditions or with phosphate ion competition, the dual affinity mechanisms of borated zirconia are confirmed. Benefiting from such unique feature, borated zirconia can function well under neutral condition. By using borated zirconia for dispersive solid phase extraction, specific capture of cis-diol-containing catecholamines, including epinephrine (E), norepinephrine (NE) and dopamine (DA), was achieved. A reliable LC-MS/MS method was established and validated for quantification of these target analytes in plasma samples after derivatisation with benzoyl chloride. The linear ranges are 0.010-0.200 ng·mL for E and DA, and 0.050-1.000 ng·mL for NE. The limits of quantification are 0.008, 0.020 and 0.004 ng·mL for E, NE and DA respectively. By analyzing samples from healthy volunteers and schizophrenia patients, the plasma concentrations of E, NE and DA were found to be higher for the latter. Graphical AbstractSchematic representation of boronate combined metal oxide affinity chromatography (BMOAC) extraction of cis-diol catecholamines from human plasma by borated zirconia following with benzoyl chloride derivatization and LC-MS determination.
硼酸亲和层析和金属氧化物亲和层析机制首次同时在一种材料上实现，这种材料称为硼酸化氧化锆。该材料是通过在非水条件下用硼酸水解锆丁醇制备的。形成的颗粒直径约为 200nm。通过在不同 pH 条件下或与磷酸盐离子竞争提取儿茶酚，证实了硼酸化氧化锆的双重亲和机制。由于这种独特的特性，硼酸化氧化锆可以在中性条件下很好地发挥作用。通过使用硼酸化氧化锆进行分散固相萃取，可以特异性捕获含有顺二醇的儿茶酚胺，包括肾上腺素（E）、去甲肾上腺素（NE）和多巴胺（DA）。建立并验证了一种可靠的 LC-MS/MS 方法，用于在衍生化苯甲酰氯后对血浆样品中的这些目标分析物进行定量。E 和 DA 的线性范围为 0.010-0.200ng·mL，NE 的线性范围为 0.050-1.000ng·mL。E、NE 和 DA 的定量限分别为 0.008、0.020 和 0.004ng·mL。通过分析来自健康志愿者和精神分裂症患者的样本，发现后者的 E、NE 和 DA 血浆浓度更高。
