Department of Biological Sciences, FFUP - Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Food Funct. 2018 Jun 20;9(6):3134-3142. doi: 10.1039/c8fo00446c.
Melissa officinalis is a plant from the family Lamiaceae, native in Europe particularly in the Mediterranean region. Given our interest in identifying extracts and compounds capable of inhibiting tumor cell growth, and given the antioxidant content and the high consumption of Melissa officinalis in Portugal, this study aimed to test the tumor cell growth inhibitory activity of five different extracts of this plant (aqueous, methanolic, ethanolic, hydromethanolic and hydroethanolic) in three human tumor cell lines: MCF-7, AGS and NCI-H460. All extracts decreased cell growth in all cell lines in a concentration-dependent manner. The ethanolic extract was the most potent one, presenting a GI50 concentration of approximately 100.9 μg mL-1 in the NCI-H460 lung cancer cells. This extract was characterized by LC-DAD-ESI/MS regarding its phenolic composition, revealing rosmarinic acid as the most abundant compound. The GI75 concentration of this extract affected the cell cycle profile of these cells. In addition, both the GI50 and the GI75 concentrations of the extract induced cellular apoptosis. Moreover, treatment of NCI-H460 cells with this extract caused a decrease in pro-caspase 3 and an increase in p53 levels. This study emphasizes the relevance of the study of natural products as inhibitors of tumor cell growth.
迷迭香是唇形科植物，原产于欧洲，特别是地中海地区。鉴于我们对鉴定能够抑制肿瘤细胞生长的提取物和化合物的兴趣，以及迷迭香在葡萄牙的抗氧化含量和高消费，本研究旨在测试这种植物的五种不同提取物（水提物、甲醇提取物、乙醇提取物、水-甲醇提取物和水-乙醇提取物）对三种人类肿瘤细胞系（MCF-7、AGS 和 NCI-H460）的肿瘤细胞生长抑制活性。所有提取物均以浓度依赖性方式降低所有细胞系中的细胞生长。乙醇提取物是最有效的提取物，在 NCI-H460 肺癌细胞中，GI50 浓度约为 100.9μgmL-1。该提取物通过 LC-DAD-ESI/MS 对其酚类成分进行了表征，表明迷迭香酸是最丰富的化合物。该提取物的 GI75 浓度影响这些细胞的细胞周期谱。此外，提取物的 GI50 和 GI75 浓度均诱导细胞凋亡。此外，用该提取物处理 NCI-H460 细胞会导致原半胱天冬酶 3 减少和 p53 水平增加。本研究强调了研究天然产物作为肿瘤细胞生长抑制剂的相关性。
