体外凝血引发抗烟曲霉中性粒细胞反应。
In vitro coagulation triggers anti-Aspergillus fumigatus neutrophil response.
机构信息
Univ Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France.
CHU Lille, Laboratoire de Parasitologie-Mycologie, F-59000 Lille, France.
出版信息
Future Microbiol. 2018 May;13:659-669. doi: 10.2217/fmb-2017-0190. Epub 2018 May 23.
AIM
To explore Aspergillus interactions with platelets in the blood, especially during clot formation.
MATERIALS & METHODS: Aspergillus fumigatus resting or swollen conidia, germlings or hyphae were inoculated into blood sampled into tubes with or without anticoagulant. Interactions were explored using microscopy, and chemokine levels were determined.
RESULTS
Anatomopathological examination of the clot revealed conidia and germlings colocalization with platelet aggregates, and neutrophil recruitment around aggregates. Transmission electron microscopy showed conidia and hyphae surrounded by neutrophils. Increased CCL5 and CXCL4 when conidia or germlings but not hyphae were added suggested they could be involved in neutrophil recruitment around aggregates.
CONCLUSION
These data suggest platelets could trigger coagulopathy and activate neutrophils during aspergillosis. They open up new perspectives for aspergillosis management.
目的
探索血液中血小板与曲霉的相互作用，尤其是在血栓形成过程中的作用。
材料与方法
将静息或肿胀的烟曲霉分生孢子、芽生孢子或菌丝接种到含有或不含有抗凝剂的采血管中的血液中。使用显微镜观察相互作用，并测定趋化因子水平。
结果
血栓的解剖病理学检查显示，分生孢子和芽生孢子与血小板聚集物以及聚集物周围的中性粒细胞募集有关。透射电子显微镜显示，被中性粒细胞包围的分生孢子和菌丝。当添加分生孢子或芽生孢子而非菌丝时，CCL5 和 CXCL4 增加，表明它们可能参与了聚集物周围中性粒细胞的募集。
结论
这些数据表明，血小板可能在曲霉病期间引发凝血功能障碍和激活中性粒细胞。它们为曲霉病的治疗开辟了新的视角。
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