de Moraes F M, Espósito D L A, Klein T M, da Fonseca B A L
Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Braz J Med Biol Res. 2018;51(6):e7221. doi: 10.1590/1414-431x20187221. Epub 2018 May 21.
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR. When tested against spiked samples, the previously described primers showed different sensitivity, with very similar results when samples from patients (serum and urine) were analyzed. Real-time RT-PCR designed to amplify Zika virus NS1 showed the best analytical sensitivity for all samples.
寨卡病毒、登革热病毒和基孔肯雅病毒感染的临床表现非常相似，仅基于临床症状很难做出诊断。此外，针对寨卡病毒的抗体与其他黄病毒之间存在强烈的交叉反应，准确诊断寨卡病毒最好采用实时逆转录聚合酶链反应（RT-PCR）。然而，一些实时RT-PCR的性能优于其他方法。为了尽可能准确地诊断寨卡病毒，我们在加标样本和临床样本中评估了一些基于探针的实时RT-PCR扩增寨卡病毒RNA的分析灵敏度。我们评估了之前发表的用于检测寨卡病毒的引物和探针，并通过实时RT-PCR使用加标血清和患者样本测试了灵敏度。在针对加标样本进行测试时，先前描述的引物表现出不同的灵敏度，分析患者（血清和尿液）样本时结果非常相似。设计用于扩增寨卡病毒NS1的实时RT-PCR对所有样本显示出最佳的分析灵敏度。
