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通过RNA测序检测婴儿血管瘤中环状RNA的表达谱。

Expression profile of circular RNAs in infantile hemangioma detected by RNA-Seq.

作者信息

Li Jun, Li Qian, Chen Ling, Gao Yanli, Li Jingyun

机构信息

Department of Plastic and Cosmetic Surgery, Maternal and Child Health Medical Institute, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China.

出版信息

Medicine (Baltimore). 2018 May;97(21):e10882. doi: 10.1097/MD.0000000000010882.

DOI:10.1097/MD.0000000000010882
PMID:29794795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6392910/
Abstract

BACKGROUND

Circular RNAs (circRNAs) have emerged as a novel class of widespread non-coding RNAs, and they play crucial roles in various biological processes. However, the characterization and function of circRNAs in infantile hemangioma (IH) remain elusive.

METHODS

In this study, we used RNA-Seq and circRNA prediction to study and characterize the circRNAs in IH tissue and a matched normal skin control. Specific circRNAs were verified using real-time polymerase chain reaction.

RESULTS AND CONCLUSION

We found that of the 9811 identified circRNAs, 249 candidates were differentially expressed, including 124 upregulated and 125 downregulated circRNAs in the IH group compared with the matched normal skin control group. A set of differentially expressed circRNAs (in particular, hsa_circRNA001885 and hsa_circRNA006612 expression) were confirmed using qRT-PCR. Gene ontology and pathway analysis revealed that compared to matched normal skin tissues, many processes that were over-represented in IH group were related to the binding, protein binding, gap junction, and focal adhesion. Specific circRNAs were associated with several micro-RNAs (miRNAs) predicted using miRanda. Altogether, our findings highlight the potential importance of circRNAs in the biology of IH and its response to treatment.

摘要

背景

环状RNA（circRNAs）已成为一类广泛存在的新型非编码RNA，在各种生物学过程中发挥关键作用。然而，circRNAs在婴儿血管瘤（IH）中的特征和功能仍不清楚。

方法

在本研究中，我们使用RNA测序和circRNA预测来研究和表征IH组织及匹配的正常皮肤对照中的circRNAs。使用实时聚合酶链反应验证特定的circRNAs。

结果与结论

我们发现，在鉴定出的9811个circRNAs中，有249个候选circRNAs差异表达，与匹配的正常皮肤对照组相比，IH组中有124个上调和125个下调的circRNAs。使用qRT-PCR证实了一组差异表达的circRNAs（特别是hsa_circRNA001885和hsa_circRNA006612的表达）。基因本体论和通路分析显示，与匹配的正常皮肤组织相比，IH组中许多过度表达的过程与结合、蛋白质结合、间隙连接和粘着斑有关。特定的circRNAs与使用miRanda预测的几种微小RNA（miRNAs）相关。总之，我们的研究结果突出了circRNAs在IH生物学及其对治疗反应中的潜在重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/23c967535ff6/medi-97-e10882-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/dd47bbc0cebf/medi-97-e10882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/1ebe9cb846ae/medi-97-e10882-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/46bc68545601/medi-97-e10882-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/fd93bdc9afe2/medi-97-e10882-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/23c967535ff6/medi-97-e10882-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/dd47bbc0cebf/medi-97-e10882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/1ebe9cb846ae/medi-97-e10882-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/46bc68545601/medi-97-e10882-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/fd93bdc9afe2/medi-97-e10882-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6451/6392910/23c967535ff6/medi-97-e10882-g009.jpg

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本文引用的文献

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2
Deep Computational Circular RNA Analytics from RNA-seq Data.基于RNA测序数据的深度计算环状RNA分析
Methods Mol Biol. 2018;1724:9-25. doi: 10.1007/978-1-4939-7562-4_2.
3
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环状RNA在儿童常见实体恶性肿瘤中的新作用
Cancer Cell Int. 2021 Jun 11;21(1):309. doi: 10.1186/s12935-021-01998-6.
4
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5
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J Clin Lab Anal. 2020 Jul;34(7):e23258. doi: 10.1002/jcla.23258. Epub 2020 Feb 24.
6
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Sci Rep. 2020 Jan 16;10(1):516. doi: 10.1038/s41598-019-57404-9.
7
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