Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China.
Department of Vascular and Hepatobilliry Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China.
J Clin Lab Anal. 2021 Mar;35(3):e23687. doi: 10.1002/jcla.23687. Epub 2021 Jan 7.
The aim of the present study was to investigate the expression profiles of circular RNAs (circRNAs) in the pericystic tissue of patients with cystic echinococcosis (CE).
CircRNA expression profiles were obtained by circRNA microarray of four matched pairs of pericystic tissues affected by CE and adjacent normal liver tissues. qRT-PCR was used to validate the differential expression of some circRNAs identified by the microarray analysis. The potential functions of the differentially expressed circRNAs in the CE pericystic tissues were predicted by bioinformatic analysis.
Compared with the adjacent normal liver tissues, 177 circRNAs were upregulated and 166 circRNAs were downregulated in CE pericystic tissues based on a ≥2.0-fold change. The top 10 upregulated circRNAs were hsa_circRNA_001654,hsa_circRNA_103361,hsa_circRNA_001490,hsa_circRNA_104310,hsa_circRNA_100395,hsa_circRNA_102485,hsa_circRNA_001459,hsa_circRNA_104193,hsa_circRNA_400043, and hsa_circRNA_006773; The top 10 downregulated circRNAs were hsa_circRNA_400633,hsa_circRNA_404974,hsa_circRNA_068482 ,hsa_circRNA_100974,hsa_circRNA_049637,hsa_circRNA_404798,hsa_circRNA_400064,hsa_circRNA_004045,hsa_circRNA_101379, and hsa_circRNA_016771;The circRNA-seq results for 15 selected differentially expressed circRNAs were validated by qRT-PCR. The qRT-PCR analysis showed that hsa_circRNA_006773, hsa_circRNA_049637, hsa_circRNA_104349, and hsa_circRNA_406281 were differentially expressed in CE pericystic tissues when compared with their expression in the adjacent normal liver tissues. Interestingly, 319 miRNAs and 52 mRNAs were predicted to be adsorbed by these four differentially expressed circRNAs. Gene Ontology analysis revealed that these circRNAs may be involved in the response to organic cyclic compounds and endogenous stimuli and in cellular organismal processes.
Differential expression of circRNAs may be associated with the development and progression of CE, and these circRNAs might be useful as biomarkers for prognosis prediction and as treatment targets.
本研究旨在探讨细粒棘球蚴（CE）囊壁组织中环状 RNA（circRNA）的表达谱。
通过对 4 对受 CE 影响的囊壁组织与相邻正常肝组织进行 circRNA 微阵列分析，获得 circRNA 表达谱。qRT-PCR 用于验证微阵列分析鉴定的部分 circRNA 的差异表达。通过生物信息学分析预测差异表达的 circRNAs 在 CE 囊壁组织中的潜在功能。
与相邻正常肝组织相比，CE 囊壁组织中 177 个 circRNA 上调，166 个 circRNA 下调，差异倍数≥2.0。上调前 10 位的 circRNA 为 hsa_circRNA_001654、hsa_circRNA_103361、hsa_circRNA_001490、hsa_circRNA_104310、hsa_circRNA_100395、hsa_circRNA_102485、hsa_circRNA_001459、hsa_circRNA_104193、hsa_circRNA_400043 和 hsa_circRNA_006773；下调前 10 位的 circRNA 为 hsa_circRNA_400633、hsa_circRNA_404974、hsa_circRNA_068482、hsa_circRNA_100974、hsa_circRNA_049637、hsa_circRNA_404798、hsa_circRNA_400064、hsa_circRNA_004045、hsa_circRNA_101379 和 hsa_circRNA_016771；对 15 个差异表达 circRNA 的 circRNA-seq 结果进行 qRT-PCR 验证。qRT-PCR 分析显示，与相邻正常肝组织相比，hsa_circRNA_006773、hsa_circRNA_049637、hsa_circRNA_104349 和 hsa_circRNA_406281 在 CE 囊壁组织中表达差异。有趣的是，这 4 个差异表达的 circRNA 可吸附 319 个 miRNA 和 52 个 mRNA。GO 分析表明，这些 circRNA 可能参与对有机环状化合物和内源性刺激的反应以及细胞生物过程。
circRNA 的差异表达可能与 CE 的发生和发展有关，这些 circRNA 可能作为预后预测的生物标志物和治疗靶点。
