Chair of Pharmacology, Jagiellonian University Medical College, Krakow, Poland.
Department of Neonatology and Intensive Care, Warsaw Medical University, Warsaw, Poland.
Pediatr Res. 2018 Jul;84(1):104-111. doi: 10.1038/s41390-018-0003-2. Epub 2018 May 23.
In this study, we aimed to analyze time-resolved plasma proteome changes in preterm neonates stratified by their gestational age to detect malfunctioning pathways that derive from the systemic immaturity of the neonate and to highlight those that are differentially regulated during the early development.
Preterm newborns were enrolled in three subgroups with different gestational ages: before 26 weeks of gestation (group 1), between 27 and 28 weeks of gestation (group 2), and between 29 and 30 (group 3) weeks of gestation. Plasma protein abundances were assessed at two time points (at preterm delivery and at the 36th week of post-menstrual age) by quantitative proteomics.
The quantitative analysis of plasma proteome in preterm infants revealed a multitude of time-related differences in protein abundances between the studied groups. We report protein changes in several functional domains, including inflammatory domains, immunomodulatory factors, and coagulation regulators as key features, with important gestational age-dependent hemopexin induction.
The global trend emerging from our data, which can collectively be interpreted as a progression toward recovery from the perinatal perturbations, highlights the profound impact of gestation duration on the ability to bridge the gap in systemic homeostasis after preterm labor.
本研究旨在分析按胎龄分层的早产儿的时间分辨血浆蛋白质组变化，以检测源自新生儿全身未成熟的功能失调途径，并强调那些在早期发育过程中差异调节的途径。
将早产儿分为三个不同胎龄亚组：<26 周（第 1 组）、27-28 周（第 2 组）和 29-30 周（第 3 组）。通过定量蛋白质组学在两个时间点（早产时和出生后 36 周）评估血浆蛋白质丰度。
早产儿血浆蛋白质组的定量分析显示，研究组之间的蛋白丰度存在多种时间相关差异。我们报告了几个功能域中的蛋白质变化，包括炎症域、免疫调节因子和凝血调节剂，这是关键特征，胎龄依赖性血色素诱导作用重要。
我们数据中出现的整体趋势可以解释为从围产期扰动中恢复的进展，突出了胎龄持续时间对早产儿分娩后系统平衡中差距的弥合能力的深远影响。
