Gao Shanshan, Xiong Wenfeng, Wei Luting, Liu Juanjuan, Liu Xing, Xie Jia, Song Xiaowen, Bi Jingxiu, Li Bin
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
Genetica. 2018 Jun;146(3):287-302. doi: 10.1007/s10709-018-0020-4. Epub 2018 May 24.
Latrophilin of Tribolium castaneum (Tclph) has been reported to play crucial roles in growth, development and reproduction. However, the regulatory mechanism of Tclph associated with these physiology processes is unknown. Thus, the global transcriptome profiles between RNAi treated (ds-Tclph) and control larvae of T. castaneum were analyzed by RNA-sequencing. Totally, 274 differentially expressed genes (DEGs) were identified between the ds-Tclph and control samples. These DEGs were classified into 42 GO functional groups, including developmental process, reproduction and stress response. The results indicated that knockdown of Tclph disturbed the antioxidant activity process, and partially inhibited the serine protease (SP) and lipase signaling pathways to regulate the development and reproduction as well as the decreasing of the stress response in T. castaneum. Additionally, knockdown of Tclph suppressed IMD immunity pathways which likely modulated the effects of Tclph on stress response. Interestingly, CSPs, ESTs, CYPs, AOXs and BGs were significantly down-regulated in ds-Tclph larvae, implying that they cooperated with Tclph to reduce the activity of cellular metabolism system. FMOs was up-regulated in ds-Tclph insects suggested it may be involved in detoxifying alkaloid of insect metabolism system. These results implied that Tclph participated in phase 0, I and II cellular detoxification. Furthermore, RNAi against Tclph increased larval susceptibility to carbamates and organophosphates insecticides, supporting that Tclph was indeed involved into the insecticide susceptibility in T. castaneum.
据报道，赤拟谷盗的促离子型受体（Tclph）在生长、发育和繁殖中发挥着关键作用。然而，Tclph与这些生理过程相关的调控机制尚不清楚。因此，通过RNA测序分析了RNA干扰处理（ds-Tclph）的赤拟谷盗幼虫和对照幼虫之间的整体转录组图谱。总共在ds-Tclph和对照样本之间鉴定出274个差异表达基因（DEG）。这些DEG被分为42个GO功能组，包括发育过程、繁殖和应激反应。结果表明，Tclph的敲低扰乱了抗氧化活性过程，并部分抑制了丝氨酸蛋白酶（SP）和脂肪酶信号通路，从而调节赤拟谷盗的发育和繁殖以及应激反应的降低。此外，Tclph的敲低抑制了IMD免疫途径，这可能调节了Tclph对应激反应的影响。有趣的是，在ds-Tclph幼虫中，化学感受蛋白（CSP）、酯酶（EST）、细胞色素P450（CYP）、抗霉素氧化酶（AOX）和β-葡萄糖苷酶（BG）显著下调，这意味着它们与Tclph协同作用以降低细胞代谢系统的活性。黄素单加氧酶（FMO）在ds-Tclph昆虫中上调，表明它可能参与昆虫代谢系统中生物碱的解毒。这些结果表明，Tclph参与了0、I和II期细胞解毒。此外，针对Tclph的RNA干扰增加了幼虫对氨基甲酸酯类和有机磷类杀虫剂的敏感性，支持Tclph确实参与了赤拟谷盗对杀虫剂的敏感性。
