Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; State Key Laboratory of Medical Genomics, Research Center for Experimental Medicine, Shanghai Rui-Jin Hospital, Department of Medical Genetics, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; College of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China.
Pestic Biochem Physiol. 2019 Sep;159:107-117. doi: 10.1016/j.pestbp.2019.06.005. Epub 2019 Jun 6.
Latrophilin (LPH) is an adhesion G protein-coupled receptor (aGPCR) that participates in multiple essential physiological processes. Our previous studies have shown that lph is not only indispensable for the development and reproduction of red flour beetles (Tribolium castaneum), but also for their resistance against dichlorvos or carbofuran insecticides. However, the regulatory mechanism of lph-mediated insecticide susceptibility remains unclear. Here, we revealed that knockdown of lph in beetles resulted in opposing changes in two chemoreception genes, chemosensory protein 10 (CSP10) and odorant-binding protein C01 (OBPC01), in which the expression of TcCSP10 was downregulated, whereas the expression of TcOBPC01 was upregulated. TcCSP10 and TcOBPC01 were expressed at the highest levels in early pupal and late larval stages, respectively. High levels of expression of both these genes were observed in the heads (without antennae) of adults. TcCSP10 and TcOBPC01 were significantly induced by dichlorvos or carbofuran between 12 and 72 h (hrs) after exposure, suggesting that they are likely associated with increasing the binding affinity of insecticides, leading to a decrease in sensitivity to the insecticides. Moreover, once these two genes were knocked down, the susceptibility of the beetles to dichlorvos or carbofuran was enhanced. Additionally, RNA interference (RNAi) targeting of lph followed by exposure to dichlorvos or carbofuran also caused the opposing expression levels of TcCSP10 and TcOBPC01 compared to the expression levels of wild-type larvae treated with insecticides alone. All these results indicate that lph is involved in insecticide susceptibility through positively regulating TcCSP10; and the susceptibility could also further partially compensated for through the negative regulation of TcOBPC01 when lph was knockdown in the red flour beetle. Our studies shed new light on the molecular regulatory mechanisms of lph related to insecticide susceptibility.
拉普罗林（LPH）是一种黏附 G 蛋白偶联受体（aGPCR），参与多种重要的生理过程。我们之前的研究表明，lph 不仅对红粉甲虫（Tribolium castaneum）的发育和繁殖是不可或缺的，而且对其对二氯氧磷或呋喃丹杀虫剂的抗性也是不可或缺的。然而，lph 介导的杀虫剂敏感性的调节机制尚不清楚。在这里，我们揭示了在甲虫中敲低 lph 会导致两种化学感受基因，化学感受蛋白 10（CSP10）和气味结合蛋白 C01（OBPC01）的表达发生相反的变化，其中 TcCSP10 的表达下调，而 TcOBPC01 的表达上调。TcCSP10 和 TcOBPC01 在早期蛹期和晚期幼虫期的表达水平最高。这两种基因在成虫的头部（无触角）表达水平最高。TcCSP10 和 TcOBPC01 在暴露后 12 至 72 小时（hrs）内被二氯氧磷或呋喃丹显著诱导，表明它们可能与增加杀虫剂的结合亲和力有关，从而降低对杀虫剂的敏感性。此外，一旦这两个基因被敲低，甲虫对二氯氧磷或呋喃丹的敏感性就会增强。此外，用 lph 的 RNA 干扰（RNAi）靶向，然后用二氯氧磷或呋喃丹处理，也会导致 TcCSP10 和 TcOBPC01 的表达水平与单独用杀虫剂处理的野生型幼虫的表达水平相反。所有这些结果表明，lph 通过正向调节 TcCSP10 参与杀虫剂敏感性；并且当 lph 在红粉甲虫中被敲低时，通过 TcOBPC01 的负向调节也可以部分补偿敏感性。我们的研究为 lph 与杀虫剂敏感性相关的分子调节机制提供了新的见解。
