Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan, China.
Pest Manag Sci. 2019 Jan;75(1):170-179. doi: 10.1002/ps.5084. Epub 2018 Aug 7.
Insects form both mutualistic and antagonistic relationships with microbes, and some antagonistic microbes have been used as biocontrol agents (BCAs) in pest management. Contextually, BCAs may be inhibited by beneficial insect symbionts, which can become potential barriers to entomopathogen-dependent pest biocontrol. Using the symbioses formed by one devastating dipteran pest, Delia antiqua, and its associated microbes as a model system, we sought to determine whether the antagonistic interaction between BCAs and microbial symbionts could affect the outcome of entomopathogen-dependent pest biocontrol.
The result showed that in contrast to non-axenic D. antiqua larvae, i.e., onion maggots, axenic larvae lost resistance to the entomopathogenic Beauveria bassiana, and the re-inoculation of microbiota increased the resistance of axenic larvae to B. bassiana. Furthermore, bacteria frequently isolated from larvae, including Citrobacter freundii, Enterobacter ludwigii, Pseudomonas protegens, Serratia plymuthica, Sphingobacterium faecium and Stenotrophomonas maltophilia, suppressed B. bassiana conidia germination and hyphal growth, and the re-inoculation of specific individual bacteria enhanced the resistance of axenic larvae to B. bassiana.
Bacteria associated with larvae, including C. freundii, E. ludwigii, P. protegens, S. plymuthica, S. faecium and S. maltophilia, can inhibit B. bassiana infection. Removing the microbiota can suppress larval resistance to fungal infection. © 2018 Society of Chemical Industry.
昆虫与微生物形成互利共生和拮抗关系，一些拮抗微生物已被用作害虫管理中的生物防治剂（BCA）。从上下文来看，BCA 可能会受到有益昆虫共生体的抑制，而这些共生体可能成为依赖昆虫病原生物防治害虫的潜在障碍。本研究以一种毁灭性双翅目害虫——地种蝇及其相关微生物的共生关系为模型系统，旨在确定 BCA 与微生物共生体之间的拮抗相互作用是否会影响依赖昆虫病原的害虫生物防治的结果。
结果表明，与非无菌地种蝇幼虫（即葱蝇）相比，无菌幼虫失去了对昆虫病原真菌球孢白僵菌的抗性，而重新接种微生物群增加了无菌幼虫对 B. bassiana 的抗性。此外，经常从幼虫中分离到的细菌，包括弗氏柠檬酸杆菌、路德维希肠杆菌、多粘类芽孢杆菌、粘质沙雷氏菌、粪肠球菌和嗜麦芽寡养单胞菌，抑制了 B. bassiana 分生孢子的萌发和菌丝生长，而重新接种特定的单个细菌增强了无菌幼虫对 B. bassiana 的抗性。
与幼虫相关的细菌，包括弗氏柠檬酸杆菌、路德维希肠杆菌、多粘类芽孢杆菌、粘质沙雷氏菌、粪肠球菌和嗜麦芽寡养单胞菌，可以抑制 B. bassiana 的感染。去除微生物群可以抑制幼虫对真菌感染的抗性。© 2018 英国化学学会。
