Zhou Fangyuan, Gao Yunxiao, Liu Mei, Xu Letian, Wu Xiaoqing, Zhao Xiaoyan, Zhang Xinjian
Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, China.
Front Microbiol. 2021 Oct 6;12:710800. doi: 10.3389/fmicb.2021.710800. eCollection 2021.
Given the multiple roles of associated microbiota in improving animal host fitness in a microbial environment, increasing numbers of researchers have focused on how the associated microbiota keeps stable under complex environmental factors, especially some biological ones. Recent studies show that associated microbiota interacts with pathogenic microbes. However, whether and how the interaction would influence microbiota stability is limitedly investigated. Based on the interaction among , its associated microbiota, and one pathogen , the associated microbiota's response to the pathogen was determined in this study. Besides, the underlying mechanism for the response was also preliminarily investigated. Results showed that neither infect larvae nor did it colonize inside the associated microbiota, and both the bacterial and fungal microbiota kept stable during the interaction. Further experiments showed that bacterial microbiota almost completely inhibited conidial germination and mycelial growth of during its invasion, while fungal microbiota did not inhibit conidial germination and mycelial growth of . According to the above results, individual dominant bacterial species were isolated, and their inhibition on conidial germination and mycelial growth of was reconfirmed. Thus, these results indicated that bacterial instead of fungal microbiota blocked conidia and stabilized the associated microbiota of larvae during invasion. The findings deepened the understanding of the role of associated microbiota-pathogen microbe interaction in maintaining microbiota stability. They may also contribute to the development of novel biological control agents and pest management strategies.
鉴于共生微生物群在微生物环境中改善动物宿主健康方面的多种作用，越来越多的研究人员关注共生微生物群在复杂环境因素，尤其是一些生物因素下如何保持稳定。最近的研究表明，共生微生物群与致病微生物相互作用。然而，这种相互作用是否以及如何影响微生物群稳定性的研究还很有限。基于[具体对象]与其共生微生物群以及一种病原体[具体病原体名称]之间的相互作用，本研究确定了共生微生物群对该病原体的反应。此外，还初步研究了这种反应的潜在机制。结果表明，[病原体名称]既不感染[具体对象]幼虫，也不在共生微生物群内定殖，并且在相互作用过程中细菌和真菌微生物群均保持稳定。进一步的实验表明，细菌微生物群在[病原体名称]入侵期间几乎完全抑制了其分生孢子萌发和菌丝生长，而真菌微生物群则没有抑制[病原体名称]的分生孢子萌发和菌丝生长。根据上述结果，分离出了单个优势细菌物种，并再次证实了它们对[病原体名称]分生孢子萌发和菌丝生长的抑制作用。因此，这些结果表明，在[病原体名称]入侵期间，是细菌而非真菌微生物群阻止了[病原体名称]分生孢子，并稳定了[具体对象]幼虫的共生微生物群。这些发现加深了对共生微生物群 - 病原体微生物相互作用在维持微生物群稳定性中作用的理解。它们也可能有助于新型生物防治剂的开发和害虫管理策略的制定。
