Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, China.
Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, China.
J Econ Entomol. 2021 Apr 13;114(2):597-610. doi: 10.1093/jee/toab002.
Recent studies have revealed multiple roles of insect-associated microbes such as lignin degradation, entomopathogen inhibition, and antibiotic production. These functions improve insect host fitness, and provide a novel source of discovering beneficial microbes for industrial and agricultural production. Previously published research found that in the symbiosis formed by the dipteran pest Delia antiqua (Meigen) (Diptera: Anthomyiidae) and its associated bacteria, the bacteria showed effective inhibition of one fungal entomopathogen, Beauveria bassiana. The antifungal activity of those associated bacteria indicates their potential to be used as biocontrol agents for fungal phytopathogens. In this study, we first isolated and identified bacteria associated with D. antiqua using a culture-dependent method. Second, we tested the antifungal activity of these bacteria against four phytopathogens including Fusarium moniliforme, Botryosphaeria dothidea, and two Fusarium oxysporum strains using the dual-culture method. In total, 74 species belonging to 30 genera, 23 families, eight classes, and four phyla were isolated and identified. Among those bacteria, Ochrobactrum anthropi, Morganella morganii, Arthrobacter sp. 3, and Acinetobacter guillouiae showed significant volatile inhibition activity against F. moniliforme, B. dothidea, and both F. oxysporum, respectively. Moreover, bacteria including Rhodococcus equi, Leucobacter aridicollis, Paenibacillus sp. 3, and Lampropedia sp. showed significant contact inhibition activity against F. moniliforme, B. dothidea, and both F. oxysporum. Our work provides a new source for discovering biocontrol agents against phytopathogens.
最近的研究揭示了昆虫相关微生物的多种作用，如木质素降解、昆虫病原体抑制和抗生素产生。这些功能提高了昆虫宿主的适应性，并为工业和农业生产中有益微生物的发现提供了新的来源。先前的研究发现，在双翅目害虫黄地老虎（Delia antiqua）（双翅目：蒼蚊科）与其相关细菌形成的共生体中，这些细菌对一种真菌性昆虫病原体白僵菌（Beauveria bassiana）表现出有效抑制作用。这些相关细菌的抗真菌活性表明它们有潜力作为真菌植物病原体的生物防治剂。在本研究中，我们首先使用培养依赖的方法从 D. antiqua 中分离和鉴定相关细菌。其次，我们使用双培养法测试了这些细菌对包括串珠镰刀菌、大丽轮枝菌和两种尖孢镰刀菌在内的四种植物病原体的抗真菌活性。总共分离和鉴定了 74 种细菌，属于 30 属、23 科、8 纲和 4 门。在这些细菌中，恶臭假单胞菌、摩氏摩根菌、节杆菌 3 号和醋酸钙不动杆菌对串珠镰刀菌、大丽轮枝菌和两种尖孢镰刀菌分别表现出显著的挥发性抑制活性。此外，包括马红球菌、解淀粉芽孢杆菌、芽孢杆菌 3 号和 Lampropedia 属在内的细菌对串珠镰刀菌、大丽轮枝菌和两种尖孢镰刀菌均表现出显著的接触抑制活性。我们的工作为发现植物病原体生物防治剂提供了新的来源。
