Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Biotechnology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
J Cell Biochem. 2018 Sep;119(9):7570-7576. doi: 10.1002/jcb.27067. Epub 2018 May 24.
Gastric cancer (GC) is among the most common cancer types in the world and one of the most lethal gastrointestinal cancers. MicroRNAs (miRNAs) can be of great importance in the early detection of GC. This study aimed to investigate some miRNAs and the genes involved in IRAK1 pathways in the serum of GC patients with Helicobacter pylori (H. pylori) infections compared to the control group. Total RNA was extracted from the serum of GC patients with H. pylori infection and healthy volunteers. The expression levels of miRNAs and the genes were assessed using Real time RT-PCR with specific primers. Our data showed that miR-146, miR-375, and Let-7 were down-regulated and miR-19 and miR-21 were up-regulated in GC patients with H. pylori infection. Other genes involved in the pathways such as RAS, MYC, NFKB, JUN, TRAF6, and IRAK4 were overexpressed; while the expression of PTEN gene was decreased compared to the control group. Expression of miRNAs and IRAK1 pathway genes are altered in patients with GC and H. pylori infection. This suggests a potential role for the above-mentioned miRNAs and genes in the diagnosis of GC.
胃癌（GC）是世界上最常见的癌症类型之一，也是最致命的胃肠道癌症之一。微小 RNA（miRNA）在 GC 的早期检测中可能具有重要意义。本研究旨在比较 H. pylori 感染的 GC 患者与对照组血清中某些 miRNA 和 IRAK1 通路相关基因的表达情况。从 H. pylori 感染的 GC 患者和健康志愿者的血清中提取总 RNA。使用特异性引物的 Real time RT-PCR 评估 miRNA 和基因的表达水平。我们的数据表明，H. pylori 感染的 GC 患者中 miR-146、miR-375 和 Let-7 下调，miR-19 和 miR-21 上调。通路中其他涉及的基因如 RAS、MYC、NFKB、JUN、TRAF6 和 IRAK4 过表达；而与对照组相比，PTEN 基因的表达减少。GC 和 H. pylori 感染患者的 miRNA 和 IRAK1 通路基因表达发生改变。这表明上述 miRNA 和基因在 GC 的诊断中可能具有潜在作用。
