Larsson S, Karlberg I, Selin E, Daneryd P, Peterson H I
Department of Physics, Chalmer's University of Technology, Göteborg, Sweden.
In Vivo. 1987 May-Jun;1(3):131-40.
Cancer cachexia is characterized by wasting of the lean tissue and profound changes in the body composition of the tumour host. These changes are partly explained by an inefficient energy production but other factors may also be important, such as deficiency of essential nutritional components. In the present study the changes of trace elements in serum and skeletal muscle were compared to those in tumour tissue during tumour progression in sarcoma--bearing rats. Trace element analysis was performed directly on serum specimens and frozen sections from skeletal muscle and tumour tissue. The samples were analysed by energy dispersive X-ray fluorescence spectrometry (EDXRF) without any other pre-treatment such as homogenization and extraction. In skeletal muscle an increased content of zinc was found during tumour progression. The iron concentration was unchanged, but since muscle wasting is part of the cachexia this means that iron was transferred to other compartments. Thus the iron content of serum was doubled and tumour tissue had a high concentration of iron. Selenium was below detection limits in skeletal muscle but well detectable in tumour tissue and it increased during tumour growth. Rubidium and potassium content correlated in all tissues (R:0.98) as did bromine and sodium (R:0.98). Copper behaved differently from the other trace elements and showed large variability. This was also true when tissue copper was individually correlated to all other trace elements in the same tissue.
癌症恶病质的特征是瘦组织消耗以及肿瘤宿主身体成分发生深刻变化。这些变化部分是由能量产生效率低下所解释的，但其他因素可能也很重要，例如必需营养成分的缺乏。在本研究中，将荷肉瘤大鼠肿瘤进展过程中血清和骨骼肌中微量元素的变化与肿瘤组织中的变化进行了比较。对血清样本以及骨骼肌和肿瘤组织的冷冻切片直接进行微量元素分析。通过能量色散X射线荧光光谱法（EDXRF）对样本进行分析，无需进行任何其他预处理，如匀浆和提取。在肿瘤进展过程中，骨骼肌中的锌含量增加。铁浓度未变，但由于肌肉消耗是恶病质的一部分，这意味着铁被转移到了其他部位。因此，血清中的铁含量增加了一倍，肿瘤组织中铁的浓度很高。骨骼肌中的硒低于检测限，但在肿瘤组织中可良好检测到，且在肿瘤生长过程中增加。铷和钾的含量在所有组织中都呈相关性（R：0.98），溴和钠也是如此（R：0.98）。铜的表现与其他微量元素不同，显示出很大的变异性。当分别将组织中的铜与同一组织中的所有其他微量元素进行相关性分析时，情况也是如此。
