Wang J S, Yan A H
Department of Otorhinolaryngology, the First Hospital of China Medical University, Shenyang, 110001, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Dec 20;31(24):1904-1909. doi: 10.13201/j.issn.1001-1781.2017.24.009.
The aim of this study is to evaluate the relationship between the expression of P16 and infection of HPV16 in sinonasal squamous cell carcinoma and its clinical and pathological features, futher prognosis was also investigated. Fifty-five cases of sinonasal squamous cell carcinoma were collected,twenty cases of nasal in-verted papilloma and twenty cases of nasal polyp were chose as control. The expression of P16 and infection of HPV16 were detected by immunohistochemistry in both experimental group and control group. The positive rate of P16 and HPV16 in sinonasal squamous cell carcinoma and nasal in-verted papilloma were significantly higher than it in nasal polyp (<0.05 or <0.01). The expression of P16 was correlated with tumor differentiation and clinical TNM stages (<0.05), but was not associated with age, sex, smoking and the primary tumor site (>0.05). HPV16 infection has no statistical relationship with analysis factors (>0.05). There was a positive correlation between P16 expression an HPV16 infection in the cases of sinonasal squamous cell carcinoma (=0.483, <0.01). Survival curves showed that the expression of P16 and infection of HPV16 were positively associated with prognosis of sinonasal squamous cell carcinoma, Log Rank test showed a significant difference (<0.05). The abnormal expression of P16 and HPV16 infection play an important role in the development of sinonasal squamous cell carcinoma. The two may had a synergistic effect. P16/HPV16 positive cases had better prognosis.
本研究旨在评估P16表达与鼻窦鳞状细胞癌中HPV16感染及其临床病理特征之间的关系，并进一步研究其预后情况。收集55例鼻窦鳞状细胞癌病例，选取20例鼻内翻性乳头状瘤和20例鼻息肉作为对照。采用免疫组织化学方法检测实验组和对照组中P16的表达及HPV16的感染情况。鼻窦鳞状细胞癌和鼻内翻性乳头状瘤中P16和HPV16的阳性率显著高于鼻息肉（<0.05或<0.01）。P16的表达与肿瘤分化及临床TNM分期相关（<0.05），但与年龄、性别、吸烟及原发肿瘤部位无关（>0.05）。HPV16感染与各分析因素无统计学关系（>0.05）。鼻窦鳞状细胞癌病例中P16表达与HPV16感染呈正相关（=0.483，<0.01）。生存曲线显示，P16表达和HPV16感染与鼻窦鳞状细胞癌的预后呈正相关，Log Rank检验显示差异有统计学意义（<0.05）。P16的异常表达和HPV16感染在鼻窦鳞状细胞癌的发生发展中起重要作用。两者可能具有协同效应。P16/HPV16阳性病例预后较好。
