环状 RNA 肌球蛋白轻链激酶（MYLK）通过调节 Mir-29a 表达促进前列腺癌进展。
Circular RNA Myosin Light Chain Kinase (MYLK) Promotes Prostate Cancer Progression through Modulating Mir-29a Expression.
机构信息
Department of Geriatric Surgery, XiangYa Hospital, Central South University, Changsha, Hunan, China (mainland).
Department of International Medical Central, XiangYa Hospital, Central South University, Changsha, Hunan, China (mainland).
出版信息
Med Sci Monit. 2018 May 25;24:3462-3471. doi: 10.12659/MSM.908009.
BACKGROUND In developed countries, prostate cancer (PCa) is a frequently diagnosed cancer with the second highest fatality rate. Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs (ncRNAs) stably expressed in cells and involved in a series of carcinomas. However, few research studies have reported on the role of circRNAs in PCa. MATERIAL AND METHODS We used qRT-PCR to detect the expression of circMYLK (circRNA ID: hsa_circ_0141940) and miR-29a in PCa tissues and cell lines. MTT, colony formation, and TUNEL assays were performed to analysis the cell viability of PCa cells. Transwell and wound scratch assays were performed to investigate the cell invasion and migration of PCa cells. RESULTS In the present study, we confirmed that circMYLK expression level was significantly higher in PCa samples and PCa cells than in normal tissues and normal prostatic cells. The upregulated circRNA-MYLK promoted PCa cells proliferation, invasion, and migration; however, si-circRNA-MYLK significantly accelerated the PCa cell apoptosis. We also observed that the aforementioned function of circRNA-MYLK on PCa cells was affected through targeting miR-29a. CONCLUSIONS We confirmed circRNA-MYLK was an oncogene in PCa and revealed a novel mechanism underlying circRNA-MYLK in PC progression.
背景
在发达国家，前列腺癌（PCa）是一种常见的癌症，死亡率居第二位。环状 RNA（circRNAs）是一类内源性非编码 RNA（ncRNAs），在细胞中稳定表达，并参与一系列癌的发生。然而，关于 circRNAs 在 PCa 中的作用的研究较少。
方法
我们使用 qRT-PCR 检测 PCa 组织和细胞系中 circMYLK（circRNA ID：hsa_circ_0141940）和 miR-29a 的表达。MTT、集落形成和 TUNEL 测定用于分析 PCa 细胞的活力。Transwell 和划痕实验用于研究 PCa 细胞的侵袭和迁移。
结果
在本研究中，我们证实 circMYLK 在 PCa 样本和 PCa 细胞中的表达水平明显高于正常组织和正常前列腺细胞。上调的 circRNA-MYLK 促进 PCa 细胞增殖、侵袭和迁移；然而，si-circRNA-MYLK 显著加速了 PCa 细胞凋亡。我们还观察到，circRNA-MYLK 对 PCa 细胞的上述功能是通过靶向 miR-29a 来实现的。
结论
我们证实 circRNA-MYLK 是 PCa 的致癌基因，并揭示了 circRNA-MYLK 在 PCa 进展中的新机制。
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