Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, QC, Canada.
Institut universitaire de cardiologie et de pneumologie de Québec, Université Laval, Québec, QC, Canada.
Food Chem Toxicol. 2018 Aug;118:390-398. doi: 10.1016/j.fct.2018.05.049. Epub 2018 May 22.
In an effort to reduce smoking-related diseases, alternative products such as e-cigarettes have been proposed. However, despite their growing popularity, the potential toxicity of e-cigarettes remains largely unknown. In this study, human gingival fibroblasts were repeatedly exposed to cigarette smoke condensate (CSC) and to nicotine-rich (NR) or nicotine-free (NF) e-vapor condensates for 60 min once a day for various time periods. They were then used to perform different analyses. Results indicate that cells exposed to CSC or NR condensates showed an altered morphology and a reduced proliferation rate, as ascertained by MTT and BrdU assays. Fibroblast cultures exposed to either CSC or e-vapor condensates also showed increased levels of TUNEL-positive apoptotic cells, compared to that recorded in the control. Furthermore, the cell scratch test revealed that repeated exposures to CSC or to e-vapor condensates delayed both fibroblast migration and wound healing. It should be noted that CSC was much more damageable to gingival fibroblasts than were the NR and NF e-vapor condensates. The representative chain of damage thus translates to CSC > NR e-vapor condensate > NF e-vapor condensate.
为了减少与吸烟有关的疾病，人们提出了电子烟等替代产品。然而，尽管它们越来越受欢迎，但电子烟的潜在毒性在很大程度上仍不清楚。在这项研究中，人牙龈成纤维细胞被反复暴露于香烟烟雾冷凝物（CSC）和富含尼古丁（NR）或无尼古丁（NF）的电子烟蒸汽冷凝物中，每天一次，每次 60 分钟，持续不同的时间。然后，用它们进行不同的分析。结果表明，与对照组相比，暴露于 CSC 或 NR 冷凝物的细胞形态发生改变，增殖率降低，这可通过 MTT 和 BrdU 检测法确定。与对照组相比，暴露于 CSC 或电子烟蒸汽冷凝物的成纤维细胞培养物中，TUNEL 阳性凋亡细胞的水平也升高。此外，细胞划痕试验表明，反复暴露于 CSC 或电子烟蒸汽冷凝物会延迟成纤维细胞的迁移和伤口愈合。值得注意的是，CSC 对牙龈成纤维细胞的损伤比 NR 和 NF 电子烟蒸汽冷凝物大得多。因此，损伤的代表性链为 CSC > NR 电子烟蒸汽冷凝物 > NF 电子烟蒸汽冷凝物。
