Grygorieva Olga, Kucharska Alicja Zofia, Piórecki Narcyz, Klymenko Svitlana, Vergun Olena, Brindza Ján
M. M. Gryshko National Botanical Gardens of Ukraine, National Academy of Sciences, Kyiv, Ukraine.
Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wrocław University of Environmental and Life Sciences, Poland.
Acta Sci Pol Technol Aliment. 2018 Apr-Jun;17(2):117-124. doi: 10.17306/J.AFS.0544.
American persimmons (Diospyros virginiana L.) are known as a widespread cultivar which were traditionally used by Native Americans as a food source, and since ancient times have been used in folk medicine. The objective of this study was to evaluate the antioxidant activity and phenolic content of Diospyros virginiana genotypes.
The content of the total antioxidant activity and phenolic compounds from the fruits of the American persimmon (Diospyros virginiana L.) of six genotypes were compared. Antioxidant activity (AOA) was measured using three different photometric methods – DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric-reducing antioxidant power). Total phenolic content (TPC) was evaluated using Folin-Ciocalteu reagent assay.
The results for AOA (μMol Trolox/g) determined by the DPPH method varied from 51.68 (DV-05) to 100.87 (DV-03), those obtained by the ABTS method varied from 65.40 (DV-05) to 142.26 (DV-03), and those obtained by the FRAP method varied from 45.06 (DV-05) to 109.30 (DV-03). The results for TPC varied from 590.75 ±27.98 mg/100 g (DV-05) to 1325.12 ±77.30 mg/100 g (DV-03). The highest results for AOA and TPC were achieved for the fruits of genotypes DV-03 and DV-05. A positive linear correlation was found between antioxidant activity and total phenolic content in the examined plant material.
The results showed that all fruit extracts exhibited strong antioxidant activities, which generally correlated positively with the total phenolic content. This study demonstrates the potential of the fruits of Diospyros virginiana grown in Ukraine as a possible source of valuable polyphenol content, with high anti- oxidant activities and health-promoting properties. The high contents of phenolic compounds and significant linear correlation between the values of the concentration of phenolic compounds and antioxidant activity indicated that these compounds contributed to the strong antioxidant activity.
美洲柿（弗吉尼亚柿，Diospyros virginiana L.）是一种广泛种植的品种，传统上被美洲原住民用作食物来源，并且自古以来就被用于民间医学。本研究的目的是评估弗吉尼亚柿不同基因型的抗氧化活性和酚类含量。
比较了六种基因型美洲柿（弗吉尼亚柿，Diospyros virginiana L.）果实的总抗氧化活性和酚类化合物含量。使用三种不同的光度法测定抗氧化活性（AOA）——DPPH（2,2-二苯基-1-苦基肼）、ABTS（2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸）和FRAP（铁还原抗氧化能力）。使用福林-酚试剂法评估总酚含量（TPC）。
通过DPPH法测定的AOA（μMol Trolox/g）结果在51.68（DV-05）至100.87（DV-03）之间，通过ABTS法获得的结果在65.40（DV-05）至142.26（DV-03）之间，通过FRAP法获得的结果在45.06（DV-05）至109.30（DV-03）之间。TPC结果在590.75 ±27.98 mg/100 g（DV-05）至1325.12 ±77.30 mg/100 g（DV-03）之间。基因型DV-03和DV-05的果实获得了最高的AOA和TPC结果。在所检测的植物材料中，抗氧化活性与总酚含量之间存在正线性相关。
结果表明，所有果实提取物均表现出较强的抗氧化活性，这通常与总酚含量呈正相关。本研究证明了在乌克兰种植的弗吉尼亚柿果实作为一种可能的有价值的多酚含量来源的潜力，其具有高抗氧化活性和促进健康的特性。酚类化合物的高含量以及酚类化合物浓度值与抗氧化活性之间的显著线性相关性表明这些化合物有助于强大的抗氧化活性。
