Forouzanfar Fatemeh, Mirdoosti Motahareh, Akaberi Maryam, Rezaee Ramin, Esmaeili Seyed-Alireza, Saburi Ehsan, Mahaki Hanie
Medical Toxicology Research Center, School of Medicine Mashhad University of Medical Sciences Mashhad Iran.
Department of Pharmacognosy, School of Pharmacy Mashhad University of Medical Sciences Mashhad Iran.
Food Sci Nutr. 2024 Aug 20;12(10):8084-8092. doi: 10.1002/fsn3.4418. eCollection 2024 Oct.
Current treatments for rheumatoid arthritis produce untoward effects; thus, considerable effort has been made to recognize effective herbal medicines against the condition. In the present study, the therapeutic effect of fruit hydroalcoholic extract (DFHE) on complete Freund's adjuvant (CFA)-induced arthritis in rats was investigated. The extract was characterized using liquid chromatography-electrospray mass spectrometry (LC-ESIMS). Male Wistar rats were grouped as follows (eight rats in each): control, CFA, CFA + indomethacin (5 mg/kg), CFA + DFHE (50 mg/kg), and CFA + DFHE (100 mg/kg). Paw volume, mechanical allodynia, thermal hyperalgesia, and arthritis score were evaluated. Serum levels of malondialdehyde (MDA), thiol groups, tumor necrosis factor-alpha (TNF-α), as well as glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were evaluated. Carotenoids were found to be the major components of DFHE. Administration of DFHE (100 mg/kg) significantly decreased arthritis score, paw volume, and thermal hyperalgesia, and improved mechanical allodynia. MDA and TNF-α levels were decreased while thiol levels and SOD and GPx activities were increased in DFHE-treated groups compared to the CFA group. These results suggest that extract caused an improvement in clinical signs of rheumatoid arthritis symptoms possibly through suppression of oxidative stress and inflammation.
类风湿性关节炎的现有治疗方法会产生不良影响；因此，人们已付出巨大努力来寻找针对该病症的有效草药。在本研究中，研究了枣果实水醇提取物（DFHE）对完全弗氏佐剂（CFA）诱导的大鼠关节炎的治疗效果。使用液相色谱 - 电喷雾质谱法（LC - ESIMS）对提取物进行了表征。将雄性Wistar大鼠分为以下几组（每组8只）：对照组、CFA组、CFA + 吲哚美辛（5 mg/kg）组、CFA + DFHE（50 mg/kg）组和CFA + DFHE（100 mg/kg）组。评估了爪体积、机械性异常性疼痛、热痛觉过敏和关节炎评分。评估了血清丙二醛（MDA）水平、硫醇基团、肿瘤坏死因子 - α（TNF - α）以及谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性。发现类胡萝卜素是DFHE的主要成分。给予DFHE（100 mg/kg）可显著降低关节炎评分、爪体积和热痛觉过敏，并改善机械性异常性疼痛。与CFA组相比，DFHE治疗组的MDA和TNF - α水平降低，而硫醇水平以及SOD和GPx活性增加。这些结果表明，该提取物可能通过抑制氧化应激和炎症改善了类风湿性关节炎症状的临床体征。
