College of Chemical Engineering, Huaqiao University, Jimei Road, Jimei District, Xiamen 361021, Fujian, PR China; Xiamen Engineering and Technological Research Center for Comprehensive Utilization of Marine Biological Resources, Jimei Road, Jimei District, Xiamen 361021, Fujian, PR China.
College of Chemical Engineering, Huaqiao University, Jimei Road, Jimei District, Xiamen 361021, Fujian, PR China.
Int J Biol Macromol. 2018 Oct 1;117:696-703. doi: 10.1016/j.ijbiomac.2018.05.171. Epub 2018 May 24.
In this study, agarose was chemically modified with ethylene oxide, 1,2-epoxypropane and 1,2-epoxybutane, to prepare hydroxyethyl agarose (HEAG), hydroxypropyl agarose (HPAG), and hydroxybutyl agarose (HBAG). The structures and properties of the products were characterized by Fourier transform infrared spectroscopy (FTIR), H NMR spectroscopy, X-ray diffractometry (XRD), rheology measurement and gel electrophoresis. The results showed that the gelling temperature (T) of HEAG, HPAG, and HBAG were decreased to 28.3 °C, 29.0 °C and 28.7 °C respectively, with the melting temperature (T) simultaneously declined to 64.2 °C, 63.8 °C and 63.0 °C, respectively. Meanwhile, the gel strength of HBAG, HEAG, and HPAG were dramatically reduced to 194 g/cm, 312 g/cm and 279 g/cm, respectively. Furthermore, HEAG and HPAG showed excellent separation efficiency which is in accordance with commercialized low-melting-point (LMP) agarose (Amersco 0815). These results indicated that oxyalkylation is a promising and economical method for the preparation of LMP agarose.
在这项研究中，琼脂糖通过环氧乙烷、1,2-环氧丙烷和 1,2-环氧丁烷进行化学修饰，制备了羟乙基琼脂糖（HEAG）、羟丙基琼脂糖（HPAG）和羟丁基琼脂糖（HBAG）。通过傅里叶变换红外光谱（FTIR）、H 核磁共振光谱（NMR）、X 射线衍射（XRD）、流变学测量和凝胶电泳对产物的结构和性能进行了表征。结果表明，HEAG、HPAG 和 HBAG 的胶凝温度（T）分别降低至 28.3°C、29.0°C 和 28.7°C，熔融温度（T）同时降低至 64.2°C、63.8°C 和 63.0°C。同时，HBAG、HEAG 和 HPAG 的凝胶强度分别急剧降低至 194 g/cm、312 g/cm 和 279 g/cm。此外，HEAG 和 HPAG 表现出优异的分离效率，与商业化的低熔点琼脂糖（Amersco 0815）一致。这些结果表明，烷氧基化是制备低熔点琼脂糖的一种有前途且经济的方法。
