Ataya K M, Pydyn E F, Sacco A G
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Reprod Toxicol. 1988;2(2):105-9. doi: 10.1016/0890-6238(88)90005-6.
To investigate the involvement of oocytes in the mechanism of chemotherapy-induced ovarian failure, ovulation was induced in mice using pregnant mares' serum gonadotropin and human chorionic gonadotropin. Oocytes (with cumulus) were incubated for 4 hours with "activated" cyclophosphamide, 4-hydroperoxycyclophosphamide (PCTX) at 0, 1, 10, 100, and 500 micrograms/mL. Oocytes were then washed, fertilized with sperm obtained from nontreated male proven breeders, and incubated for 4 days. "Activated" cyclophosphamide inhibited dissolution of the cumulus and reduced fertilization and early cleavage rates in a dose-related manner. The data demonstrate that exposure of oocytes to cyclophosphamide metabolites in vitro adversely affects oocyte function. Oocytes may be involved in the mechanism of cyclophosphamide-induced ovarian failure.
为研究卵母细胞在化疗诱导的卵巢功能衰竭机制中的作用，使用孕马血清促性腺激素和人绒毛膜促性腺激素诱导小鼠排卵。将卵母细胞（带卵丘）与浓度为0、1、10、100和500微克/毫升的“活化”环磷酰胺、4-氢过氧环磷酰胺（PCTX）一起孵育4小时。然后冲洗卵母细胞，用未经处理的雄性种公鼠的精子使其受精，并孵育4天。“活化”环磷酰胺以剂量相关的方式抑制卵丘溶解，并降低受精率和早期卵裂率。数据表明，卵母细胞在体外暴露于环磷酰胺代谢产物会对卵母细胞功能产生不利影响。卵母细胞可能参与环磷酰胺诱导的卵巢功能衰竭机制。
