Alsalili M, Thornton S, Fleming S
Department of Obstetrics and Gynaecology, University Hospital, Nottingham, UK.
Hum Reprod. 1997 Jun;12(6):1271-4. doi: 10.1093/humrep/12.6.1271.
Propofol is a common anaesthetic agent used for oocyte retrieval procedures during in-vitro fertilization (IVF). The effect of Propofol in vitro on mouse oocyte maturation, fertilization and embryo cleavage was studied. In this study, 551 cumulus-free and 222 cumulus-enclosed oocytes from mice stimulated with pregnant mare's serum gonadotrophin (PMSG) were incubated for 30 min in medium containing 0, 100, 1000 or 10,000 ng/ml of Propofol prior to in-vitro maturation. Also, 325 cumulus-enclosed oocytes from mice stimulated to ovulate with PMSG/human chorionic gonadotrophin (HCG) were incubated for 30 min in similar concentrations of Propofol prior to IVF. Maturation, fertilization and cleavage rates were compared. A significant decrease in the in-vitro maturation rate was observed only when the cumulus-free and cumulus-enclosed oocytes were exposed to 10,000 ng/ml Propofol (P < 0.0074 and P < 0.0001 respectively). Fertilization and embryo cleavage rates were not significantly different compared with the controls. These findings give some reassurance with respect to human IVF. However, further studies on the potential effects of Propofol on implantation and pregnancy outcome following IVF are needed.
丙泊酚是一种常用于体外受精（IVF）过程中取卵手术的麻醉剂。研究了丙泊酚在体外对小鼠卵母细胞成熟、受精和胚胎分裂的影响。在本研究中，将551个来自用孕马血清促性腺激素（PMSG）刺激的小鼠的裸卵和222个卵丘包裹的卵母细胞在体外成熟前于含有0、100、1000或10000 ng/ml丙泊酚的培养基中孵育30分钟。此外，将325个来自用PMSG/人绒毛膜促性腺激素（HCG）刺激排卵的小鼠的卵丘包裹的卵母细胞在体外受精前于类似浓度的丙泊酚中孵育30分钟。比较了成熟、受精和分裂率。仅当裸卵和卵丘包裹的卵母细胞暴露于10000 ng/ml丙泊酚时，体外成熟率才显著降低（分别为P < 0.0074和P < 0.0001）。与对照组相比，受精和胚胎分裂率无显著差异。这些发现为人类IVF提供了一些保证。然而，需要进一步研究丙泊酚对IVF后着床和妊娠结局的潜在影响。
