Anwar M, Beck F
Department of Anatomy, University of Leicester, England.
Reprod Toxicol. 1988;2(2):135-40. doi: 10.1016/0890-6238(88)90009-3.
The normal growth of rat embryos cultured in human serum supplemented with glucose and 10% rat serum is of potential use in the assessment of teratogenic risk in man. Before this technique can be widely applied, it is necessary to know whether the stages of the menstrual cycle at which a serum sample is tested materially affect the results obtained. Moreover, in order to achieve reproducible conditions we have found it necessary to add a minimal amount of rat serum to the human serum used for culture, but unnecessarily high levels of rat serum supplementation could over-compensate for any growth factor deficiency in human serum. Here we report that culture of rat conceptuses gives similar results irrespective of whether human first, second, or third trimester pregnancy serum, postnatal serum, or serum at various stages of the menstrual cycle is used. We also report that addition of 2% rat serum supplement is sufficient to achieve reproducible rat embryonic growth and differentiation in human serum.
在添加葡萄糖和10%大鼠血清的人血清中培养的大鼠胚胎的正常生长，在评估人类致畸风险方面具有潜在用途。在该技术能够广泛应用之前，有必要了解检测血清样本时的月经周期阶段是否会实质性地影响所获得的结果。此外，为了实现可重复的条件，我们发现有必要向用于培养的人血清中添加少量的大鼠血清，但过高水平的大鼠血清补充可能会过度补偿人血清中任何生长因子的缺乏。在此我们报告，无论使用人类孕早期、孕中期或孕晚期血清、产后血清还是月经周期各阶段的血清，大鼠孕体的培养都能得到相似的结果。我们还报告，添加2%的大鼠血清补充剂足以在人血清中实现可重复的大鼠胚胎生长和分化。
