Institut de Recherche Clinique de Montréal, 110 Avenue des Pins, H2W 1R7, Montréal, Québec, Canada.
Department of Medicine, Université de Montréal, 2900, boul. Édouard-Montpetit, Montréal, Québec, H3T 1J4, Canada.
Inflammation. 2018 Oct;41(5):1631-1639. doi: 10.1007/s10753-018-0806-7.
The chitinase-3-like protein 1, also known as YKL-40, is an inflammatory marker increased in blood of patients with cystic fibrosis (CF). Systemic levels of YKL-40 are increased in dysglycemic patients with CF. Our objective is to determine if YKL-40 is expressed and released by CF neutrophils. We also assessed if specific stimulus, such as glucose and lipopolysaccharide (LPS), can induce the secretion of YKL-40. Neutrophil cells of healthy adults and patients with CF were isolated. Immunostaining of whole blood and neutrophils was done. CF and healthy neutrophils were cultured with either LPS or varying concentrations of glucose. YKL-40 protein was measured using specific immunoassay ELISA. Isolated neutrophil cells from 11 patients with CF (32.3 ± 8.0 years) were compared to five age-matched healthy individuals (28.3 ± 5.5 years). Although there is a significant increase in the concentration of YKL-40 in CF neutrophils compared to healthy neutrophils (P = 0.027), the spontaneous release of YKL-40 into the media is similar in CF and healthy neutrophils. CF neutrophils stimulated with LPS or glucose do not stimulate the release of YKL-40 (P = 0.995 for glucose and P = 0.624 for LPS). CF neutrophils have higher intracellular level of YKL-40 than neutrophils from healthy volunteers, but they do not release more YKL-40 when stimulated with exogenous stimulus. These results suggest that the increased levels of circulating YKL-40 in CF patients might originate from another cellular source.
几丁质酶 3 样蛋白 1，也称为 YKL-40，是囊性纤维化 (CF) 患者血液中增加的炎症标志物。患有 CF 的糖尿病患者血液中的全身性 YKL-40 水平升高。我们的目的是确定 YKL-40 是否由 CF 中性粒细胞表达和释放。我们还评估了特定刺激物（如葡萄糖和脂多糖 (LPS)）是否可以诱导 YKL-40 的分泌。分离健康成年人和 CF 患者的中性粒细胞。进行全血和中性粒细胞的免疫染色。用 LPS 或不同浓度的葡萄糖培养 CF 和健康的中性粒细胞。使用特定的免疫测定 ELISA 测量 YKL-40 蛋白。将 11 名 CF 患者（32.3 ± 8.0 岁）的分离中性粒细胞与 5 名年龄匹配的健康个体（28.3 ± 5.5 岁）进行比较。尽管 CF 中性粒细胞中的 YKL-40 浓度与健康中性粒细胞相比显着增加（P = 0.027），但 CF 和健康中性粒细胞中 YKL-40 的自发释放相似。用 LPS 或葡萄糖刺激 CF 中性粒细胞不会刺激 YKL-40 的释放（P = 0.995 用于葡萄糖，P = 0.624 用于 LPS）。CF 中性粒细胞的细胞内 YKL-40 水平高于健康志愿者的中性粒细胞，但在用外源性刺激物刺激时不会释放更多的 YKL-40。这些结果表明，CF 患者循环 YKL-40 水平升高可能源自另一种细胞来源。
