Cai Jiangyu, Jiang Jia, Mo Xiumei, Chen Shiyi
Department of Sports Medicine and Arthroscopic Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, P.R.China.
Biomaterials and Tissue Engineering Laboratory, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, P.R.China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017 Aug 15;31(8):957-962. doi: 10.7507/1002-1892.201704077.
To explore the effect of silk fibroin/poly( -lactic acid-co-e-caprolactone) [SF/P(LLA-CL)] nanofibrous scaffold on tendon-bone healing of rabbits.
SF/P(LLA-CL) nanofibrous scaffold was fabricated by electrospinning methods. The morphology of the scaffold was observed by scanning electron microscope (SEM). Pre-osteoblasts MC3T3-E1 cells were seeded on the scaffold and cultured for 1, 3, and 5 days. Cell adhesion and proliferation were also observed by SEM. Meanwhile, twenty-four New Zealand white rabbits were randomly divided into the autogenous tendon group (control group) and the autogenous tendon wrapped with SF/P(LLA-CL) scaffold group (experimental group), with twelve rabbits in each group. An extra-articular model was established, the effect was evaluated by histological examination and mechanical testing.
The morphology of SF/P(LLA-CL) nanofibrous scaffold was random, with a diameter of (219.4±66.5) nm. SEM showed that the MC3T3-E1 cells seeded on the scaffold were in the normal shape, growing well, and proliferating with time course. The results of histological examination showed that inflammatory cells infltrated into the graft-host bone interface at 6 weeks after operation in both groups. Besides, the width of interface showed no significant difference between groups. At 12 weeks after operation, protruding new bone tissue could be observed at the interface in the experimental group, while scar tissue but no new bone tissue could be seen at the interface in the control group. Mechanical testing showed that there was no significant difference in the failure load and the stiffness between groups at 6 weeks after operation ( >0.05). The failure load and the stiffness in the experimental group were significantly higher than those in the control group at 12 weeks after operation ( <0.05).
The SF/P(LLA-CL) nanofibrous scaffold has good cell biocompatibility and can effectively promote tendon-bone healing, thus providing new method for modifying graft for ACL reconstruction in the clinical practice.
探讨丝素蛋白/聚（L-乳酸-共-己内酯）[SF/P(LLA-CL)]纳米纤维支架对兔肌腱-骨愈合的影响。
采用静电纺丝法制备SF/P(LLA-CL)纳米纤维支架。通过扫描电子显微镜（SEM）观察支架的形态。将前成骨细胞MC3T3-E1细胞接种于支架上，培养1、3和5天。同时通过SEM观察细胞黏附和增殖情况。此外，将24只新西兰白兔随机分为自体肌腱组（对照组）和自体肌腱包裹SF/P(LLA-CL)支架组（实验组），每组12只。建立关节外模型，通过组织学检查和力学测试评估效果。
SF/P(LLA-CL)纳米纤维支架形态不规则，直径为(219.4±66.5)nm。SEM显示接种于支架上的MC3T3-E1细胞形态正常，生长良好，并随时间进程增殖。组织学检查结果显示，两组术后6周均有炎性细胞浸润至移植物-宿主骨界面。此外，两组间界面宽度无显著差异。术后12周，实验组界面可见突出的新骨组织，而对照组界面可见瘢痕组织但无新骨组织。力学测试显示，术后6周两组间的破坏载荷和刚度无显著差异（P>0.05）。术后12周，实验组的破坏载荷和刚度显著高于对照组（P<0.05）。
SF/P(LLA-CL)纳米纤维支架具有良好的细胞生物相容性，能有效促进肌腱-骨愈合，为临床前交叉韧带重建移植物的改良提供了新方法。
