Li Qiwei, Li Chaojing, Wang Fujun, Hu Sihan, Wang Lu
Key Laboratory of Textile Science & Technology, Ministry of Education, Textile College of Donghua University, Shanghai, 201620, P.R.China.
Key Laboratory of Textile Science & Technology, Ministry of Education, Textile College of Donghua University, Shanghai, 201620,
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019 Apr 15;33(4):479-485. doi: 10.7507/1002-1892.201808128.
To prepare hierarchically structured fibrous scaffolds with different morphologies, and to explore the additional dimensionality for tuning the physicochemical properties of the scaffolds and the effect of their hemocompatibility and cytocompatibility.
Electrospinning poly (e-caprolactone) (PCL)/polyvinylpyrrolidone (PVP) bicomponent fibers (PCL∶PVP mass ratios were 8∶2 and 5∶5 respectively), and the surface porous fibrous scaffolds were prepared by extracting PVP components. The scaffolds were labeled PCL-P8 and PCL-P5 respectively according to the mass ratio of polymer. In addition, shish-kebab (SK) structured scaffolds with different kebab sizes were created by solution incubation method, which use electrospun PCL fibers as shish while PCL chains in solution crystallizes on the fiber surface. The PCL fibrous scaffolds with smooth surface was established as control group. The hierarchically structured fibrous scaffolds were characterized by field emission scanning electron microspore, water contact angle tests, and differential scanning calorimeter (DSC) experiments. The venous blood of New Zealand white rabbits was taken and hemolysis and coagulation tests were used to characterize the blood compatibility of the scaffolds. The proliferation of the pig iliac artery endothelial cell (PIEC) on the scaffolds was detected by cell counting kit 8 (CCK-8) method, and the biocompatibility of the scaffolds was evaluated.
Field emission scanning electron microscopy showed that porous morphology appeared on the surface of PCL/PVP bicomponent fibers after extracting PVP. In addition, SK structure with periodic arrangement was successfully prepared by solution induction, and the longer the crystallization time, the larger the lamellar size and periodic distance. The contact angle and DSC measurements showed that when compared with smooth PCL fiber scaffolds, the crystallinity of PCL surface porous fibrous scaffolds and PCL-SK fibrous scaffolds increased, while the hydrophobicity of PCL-SK fibrous scaffolds increased, but the hydrophobicity of PCL porous scaffolds did not change significantly. The hemolysis test showed that the hemolysis rate of PCL surface porous fibrous scaffolds and PCL-SK fibrous scaffolds was higher than that of PCL fibrous scaffolds. According to American Society of Materials and Tests (ASTM) F756-08 standard, all scaffolds were non-hemolytic materials and were suitable for blood contact materials. Coagulation test showed that the coagulation index of PCL surface porous fibrous scaffolds and PCL-SK fibrous scaffolds was higher than that of PCL fibrous scaffolds at 5 and 10 minutes of culture. CCK-8 assay showed that both hierarchically structured fibrous scaffolds were more conducive to PIEC proliferation than PCL fibrous scaffold.
Based on electrospinning technology, solution-induced and blend phase separation methods can be used to construct multi-scale fiber scaffolds with different morphologies, which can not only regulate the surface physicochemical properties of the scaffolds, but also have good blood compatibility and biocompatibility. The hierarchically structured fibrous scaffolds have high application potential in the field of tissue engineering.
制备具有不同形态的分层结构纤维支架，探索调整支架物理化学性质的额外维度及其血液相容性和细胞相容性的影响。
静电纺丝制备聚（ε-己内酯）（PCL）/聚乙烯吡咯烷酮（PVP）双组分纤维（PCL∶PVP质量比分别为8∶2和5∶5），通过萃取PVP组分制备表面多孔纤维支架。根据聚合物质量比，将支架分别标记为PCL-P8和PCL-P5。此外，采用溶液孵育法制备了具有不同串珠尺寸的串珠-串晶（SK）结构支架，以静电纺PCL纤维为串晶，溶液中的PCL链在纤维表面结晶。将表面光滑的PCL纤维支架作为对照组。通过场发射扫描电子显微镜、水接触角测试和差示扫描量热仪（DSC）实验对分层结构纤维支架进行表征。采集新西兰白兔静脉血，采用溶血和凝血试验表征支架的血液相容性。采用细胞计数试剂盒8（CCK-8）法检测猪髂动脉内皮细胞（PIEC）在支架上的增殖情况，评估支架的生物相容性。
场发射扫描电子显微镜显示，萃取PVP后PCL/PVP双组分纤维表面出现多孔形态。此外，通过溶液诱导成功制备了具有周期性排列的SK结构，结晶时间越长，片层尺寸和周期距离越大。接触角和DSC测量结果表明，与光滑PCL纤维支架相比，PCL表面多孔纤维支架和PCL-SK纤维支架的PCL结晶度增加，PCL-SK纤维支架的疏水性增加，但PCL多孔支架的疏水性变化不明显。溶血试验表明，PCL表面多孔纤维支架和PCL-SK纤维支架的溶血率高于PCL纤维支架。根据美国材料与试验协会（ASTM）F756-08标准，所有支架均为非溶血材料，适用于血液接触材料。凝血试验表明，在培养5分钟和10分钟时，PCL表面多孔纤维支架和PCL-SK纤维支架的凝血指数高于PCL纤维支架。CCK-8检测表明，两种分层结构纤维支架均比PCL纤维支架更有利于PIEC增殖。
基于静电纺丝技术，采用溶液诱导和共混相分离方法可构建具有不同形态的多尺度纤维支架，不仅能调控支架表面物理化学性质，还具有良好的血液相容性和生物相容性。分层结构纤维支架在组织工程领域具有较高的应用潜力。
