Jagiellonian University Medical College, Krakow, Poland.
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK.
Environ Toxicol Pharmacol. 2018 Jul;61:24-29. doi: 10.1016/j.etap.2018.05.011. Epub 2018 May 22.
The influence of prenatal alcohol exposure on the serotoninergic system in the brain has been well studied, however its influence on the serotoninergic system in the gastrointestinal system remains unknown. The objective of the study was to use a mouse model of prenatal alcohol exposure to investigate the effects on serotonin and its metabolites and precursors in colonic tissue. This study used treatment of mouse breeding harems with 5% ethanol with saccharin via drinking water throughout pregnancy and compared the results with a saccharin control group. Tryptophan, serotonin (5-HT) and 5- hydroxyindoleacetic acid (5-HIAA) concentrations were measured in the longitudinal muscle myenteric plexus (LMMP) and mucosa of intestinal tissue by high-performance liquid chromatography (HPLC). Decreased 5-HT concentrations in mucosa and LMMP (females only) were observed in prenatally exposed mice compared to controls. Increases in mucosal and LMMP tryptophan concentration were only observed in prenatally exposed female mice. In conclusion, prenatal alcohol exposure causes a decrease in conversion of tryptophan to 5-HT in both muscle and mucosa although the effect is more pronounced in females. The observed sex difference may be related to changes associated with the estrous cycle.
产前酒精暴露对大脑中血清素能系统的影响已经得到了充分研究，然而，其对胃肠道系统中血清素能系统的影响尚不清楚。本研究的目的是使用产前酒精暴露的小鼠模型，研究其对结肠组织中血清素及其代谢物和前体的影响。本研究通过饮用水对繁殖母鼠进行 5%乙醇和糖精处理，以在整个怀孕期间进行处理，并将结果与糖精对照组进行比较。通过高效液相色谱法（HPLC）测量肠道组织的纵行肌肌间神经丛（LMMP）和黏膜中的色氨酸、血清素（5-HT）和 5-羟吲哚乙酸（5-HIAA）浓度。与对照组相比，产前暴露的小鼠的黏膜和 LMMP 中的 5-HT 浓度降低（仅雌性）。仅在产前暴露的雌性小鼠中观察到黏膜和 LMMP 色氨酸浓度增加。总之，产前酒精暴露导致色氨酸向 5-HT 的转化在肌肉和黏膜中均减少，尽管在雌性中更为明显。观察到的性别差异可能与与发情周期相关的变化有关。
