通过过表达乌桕（Sapium sebiferum （L.） Roxb.）中的硬脂酰-ACP 脱饱和酶基因（SAD）来提高油菜（Brassica napus L.）的抗冻性。
Enhancing freezing tolerance of Brassica napus L. by overexpression of a stearoyl-acyl carrier protein desaturase gene (SAD) from Sapium sebiferum (L.) Roxb.
机构信息
College of Bioscience and Biotechnology of Central South University of Forestry and Technology, 410018, Changsha, China; Forestry Biotechnology Hunan Key Laboratories, Hunan Changsha, 410018, China.
College of Bioscience and Biotechnology of Central South University of Forestry and Technology, 410018, Changsha, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018, Changsha, China; Collaborative Innovation Central of Cultivation and Utilization for Non-Wood Forest Tree Central South University of Forestry and Technology, 410018, Changsha, China; Forestry Biotechnology Hunan Key Laboratories, Hunan Changsha, 410018, China.
出版信息
Plant Sci. 2018 Jul;272:32-41. doi: 10.1016/j.plantsci.2018.03.028. Epub 2018 Apr 11.
Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W10 by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L.
乌桕（Sapium sebiferum (L.) Roxb.）是中国重要的木本油料树种和传统药用植物。酰基辅酶 A 去饱和酶（SAD）是一种脱氢酶，在油脂中饱和脂肪酸向不饱和脂肪酸的转化过程中起关键作用；这些脂肪酸极大地影响植物的抗冻性。然而，SsSAD 在乌桕中的表达是否能调节其抗冻性尚不清楚。我们的研究表明，SsSAD 在乌桕中的表达在冷冻胁迫下增加。为了进一步证实这一结果，我们构建了 pEGAD-SsSAD 载体，并通过农杆菌介导的转化将其转入油菜（B. napus L. W10）中。过表达 SsSAD 基因的转基因植物表现出明显更高的亚油酸（18:2）和亚麻酸（18:3）含量和更高的抗冻性。这些结果表明，SsSAD 在油菜中的过表达可以增加亚油酸（18:2）和亚麻酸（18:3）等多不饱和脂肪酸（PUFAs）的含量，这可能对提高油菜植物的抗冻性至关重要。因此，SsSAD 的过表达可能有助于生产抗冻性油菜品种。
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