Department of Biological Sciences, Faculty of Sciences, University of Bamenda, Bamenda, Cameroon.
Department of Biochemistry, Faculty of Sciences, University of Dschang, Dschang, Cameroon.
Am J Med Sci. 2018 Jul;356(1):56-63. doi: 10.1016/j.amjms.2018.02.006. Epub 2018 Feb 17.
Macrophages are the first cells to recognize invading foreign bodies and are central to cell mediated and humoral immunity. Therefore, the activation of macrophages is a key event for effective innate and adaptive immunity. Pterocarpus erinaceus has been reported to control infectious diseases, but the mechanism remains to be elucidated. In this study, we demonstrated the immune-modulatory effect of aqueous extract of P. erinaceus using human macrophages and lymphocytes, as well as mice.
Hot water was used to extract P. erinaceus from the stem bark. Its effect on lymphocytes was measured by evaluating proliferative response and delayed hypersensitivity. Phagocytic activity of macrophages were measured based on neutral red uptake assay, nitric oxide production and myeloperoxidase and phosphatase acid activity. Hematopoietic and infectious activities were analyzed using the effect on infectious stress and chloramphenicol-induced leucopenic mice model.
Aqueous extract showed stronger stimulatory effects on the neutral red uptake, production of nitric oxide and phosphatase acid activity in lipopolysaccharide-activated macrophages. In addition, aqueous extract significantly stimulated the proliferation of phytohemagglutinin-activated lymphocytes, enhanced delayed hypersensitivity response to erythrocytes and attenuated infection-induced fever. Furthermore, aqueous extract also significantly increased the rate of recovery of white blood cell levels in chloramphenicol-induced leucopenia mice.
The results suggest that aqueous extract of P. erinaceus stem bark is able to modulate the immune system and has potential effects in clinical conditions when an immune-enhancing and anti-infectious effect is desired.
巨噬细胞是最先识别入侵异物的细胞，是细胞介导和体液免疫的核心。因此，巨噬细胞的激活是有效固有和适应性免疫的关键事件。鸡翅木已被报道能控制传染病，但机制尚不清楚。在这项研究中，我们使用人巨噬细胞和淋巴细胞以及小鼠证明了鸡翅木水提物的免疫调节作用。
热水从树皮中提取鸡翅木。通过评估增殖反应和迟发型超敏反应来测量其对淋巴细胞的作用。通过中性红摄取测定、一氧化氮产生和髓过氧化物酶和酸性磷酸酶活性来测量巨噬细胞的吞噬活性。使用对感染应激和氯霉素诱导的白细胞减少症小鼠模型的影响来分析造血和感染活性。
水提物在脂多糖激活的巨噬细胞中对中性红摄取、一氧化氮产生和酸性磷酸酶活性的刺激作用更强。此外，水提物还显著刺激植物血凝素激活的淋巴细胞增殖，增强对红细胞的迟发型超敏反应，并减轻感染引起的发热。此外，水提物还显著提高了氯霉素诱导的白细胞减少症小鼠白细胞水平恢复的速度。
结果表明，鸡翅木树皮的水提物能够调节免疫系统，在需要免疫增强和抗感染作用的临床情况下具有潜在的效果。
