Noufou Ouédraogo, Anne-Emmanuelle Hay, Claude W Ouédraogo Jean, Richard Sawadogo W, André Tibiri, Marius Lompo, Jean-Baptiste Nikiema, Jean Koudou, Marie-Genevieve Dijoux-Franca, Pierre Guissou Innocent
Département de médecine-pharmacopée traditionnelle/pharmacie (IRSS/CNRST) 03 BP 7192 Ouagadougou 03, Burkina Faso.
Laboratoire de développement du médicament, UFR/SDS, Université de Ouagadougou 03 BP 7021 Ouagadougou 03, Burkina Faso.
Afr J Tradit Complement Altern Med. 2016 Nov 23;14(1):187-195. doi: 10.21010/ajtcam.v14i1.21. eCollection 2017.
Poir. belonging to Fabacae familly is used as medicinal plant in Burkina Faso's folk medicine. Roots of are used to treat ulcer, stomach ache and inflammatory diseases. The objective of the present study was to carry out phytochemical composition of methanol (MeOH) and dichloromethane (DCM) extracts from roots, to isolate pure compounds, and to evaluate their pharmacological activities.
Chromatographic fractionation led to the isolation of active components of the extracts. The structures were established by NMR analysis and comparison with data from literature. The anti-inflammatory activity was evaluated using croton oil-induced edema of mice ear as well as the effect of extracts against lipoxygenase and lipid peroxidation was evaluated. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Cupric-reducing antioxidant capacity (CUPRAC) methods were used to evaluate the antioxidant activity of the extracts.
Friedelin (1), 3a-hydroxyfriedelan-2-one (2), a-sophoradiol (3) and stigmasterol (4) were isolated from DCM extract and maltol-6--apiofuranoside-glucopyranoside (5) isolated from MeOH. DCM extract and friedelin, 3a-hydroxyfriedelan-2-one, a-sophoradiol showed a significant anti-inflammatory effect against ear edema. Friedelin (1), α-sophoradiol (3) and maltol-6--apiofuranoside-glucopyranoside (5) exhibited lipoxygenase inhibition. MeOH extract (100 μg/mL) inhibited lipoxygenase and lipid peroxidation activities at 45.1 ± 3% and 30.7 ± 0.5% respectively. MeOH extract, ethyl acetate fraction and butanol fraction exhibited antioxidant property with both two methods used.
The results suggested that the extracts and compounds from roots of possessed local anti-inflammatory effect, antioxidant properties and inhibitor effect against lipoxygenase and lipid peroxidation activities.
属于豆科的Poir.在布基纳法索的民间医学中被用作药用植物。其根用于治疗溃疡、胃痛和炎症性疾病。本研究的目的是对Poir.根的甲醇（MeOH）和二氯甲烷（DCM）提取物进行植物化学成分分析，分离出纯化合物，并评估其药理活性。
通过色谱分离法分离提取物的活性成分。通过核磁共振分析并与文献数据比较确定结构。使用巴豆油诱导的小鼠耳水肿评估抗炎活性，并评估提取物对脂氧合酶和脂质过氧化的影响。采用2,2-二苯基-1-苦基肼（DPPH）和铜离子还原抗氧化能力（CUPRAC）方法评估提取物的抗氧化活性。
从DCM提取物中分离出了木栓酮（1）、3a-羟基木栓烷-2-酮（2）、α-槐二醇（3）和豆甾醇（4），从MeOH提取物中分离出了麦芽酚-6'-芹菜呋喃糖苷-葡萄糖苷（5）。DCM提取物以及木栓酮、3a-羟基木栓烷-2-酮、α-槐二醇对耳水肿显示出显著的抗炎作用。木栓酮（1）、α-槐二醇（3）和麦芽酚-6'-芹菜呋喃糖苷-葡萄糖苷（5）表现出脂氧合酶抑制作用。MeOH提取物（100μg/mL）分别以45.1±3%和30.7±0.5%的抑制率抑制脂氧合酶和脂质过氧化活性。MeOH提取物、乙酸乙酯馏分和正丁醇馏分在两种方法中均表现出抗氧化性能。
结果表明，Poir.根的提取物和化合物具有局部抗炎作用、抗氧化性能以及对脂氧合酶和脂质过氧化活性的抑制作用。
