Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, 55181-83111, Iran.
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet 220, 2800, Kgs. Lyngby, Denmark.
Appl Microbiol Biotechnol. 2018 Jul;102(14):5925-5938. doi: 10.1007/s00253-018-9099-x. Epub 2018 May 28.
Oleaginous yeast Yarrowia lipolytica is an important industrial host for the production of enzymes, oils, fragrances, surfactants, cosmetics, and pharmaceuticals. More recently, improved synthetic biology tools have allowed more extensive engineering of this yeast species, which lead to the production of non-native metabolites. In this review, we summarize the recent advances of genome editing tools for Y. lipolytica, including the application of CRISPR/Cas9 system and discuss case studies, where Y. lipolytica was engineered to produce various non-native chemicals: short-chain fatty alcohols and alkanes as biofuels, polyunsaturated fatty acids for nutritional and pharmaceutical applications, polyhydroxyalkanoates and dicarboxylic acids as precursors for biodegradable plastics, carotenoid-type pigments for food and feed, and campesterol as a precursor for steroid drugs.
产油酵母解脂耶氏酵母是用于生产酶、油、香料、表面活性剂、化妆品和药物的重要工业宿主。最近，改进的合成生物学工具允许对该酵母物种进行更广泛的工程改造，从而导致非天然代谢物的产生。在这篇综述中，我们总结了用于解脂耶氏酵母的基因组编辑工具的最新进展，包括 CRISPR/Cas9 系统的应用，并讨论了工程改造解脂耶氏酵母生产各种非天然化学品的案例研究：作为生物燃料的短链脂肪醇和链烷烃、用于营养和药物应用的多不饱和脂肪酸、作为可生物降解塑料前体的聚羟基烷酸酯和二羧酸、用于食品和饲料的类胡萝卜素型色素、以及用作甾体药物前体的菜油甾醇。
