Lourenço Maria Angélica Martins, Braz Mariana Gobbo, Aun Aline Garcia, Pereira Bruna Letícia Buzati, Figueiredo Amanda Menezes, da Silva Renata Aparecida Cândido, Kazmarek Elisa Moya, Alegre Patrícia Helena Correa, Bachiega Tatiana Fernanda, Zanati Silmeia Garcia, Azevedo Paula Schmidt, Polegato Bertha Furlan, Fernandes Ana Angélica Henrique, de Paiva Sergio Alberto Rupp, Zornoff Leonardo Antonio Mamede, Minicucci Marcos Ferreira
Internal Medicine Department, Botucatu Medical School, UNESP-São Paulo State University, Botucatu, Brazil.
Department of Anesthesiology, Botucatu Medical School, UNESP-São Paulo State University, Botucatu, Brazil.
J Cell Mol Med. 2018 Aug;22(8):3996-4004. doi: 10.1111/jcmm.13683. Epub 2018 May 29.
The objective of this study was to investigate the influence of Spondias mombin (SM) supplementation on the cardiac remodelling process induced by exposure to tobacco smoke (ETS) in rats. Male Wistar rats were divided into 4 groups: group C (control, n = 20) comprised animals not exposed to cigarette smoke and received standard chow; group ETS (n = 20) comprised animals exposed to cigarette smoke and received standard chow; group ETS100 (n = 20) received standard chow supplemented with 100 mg/kg body weight/d of SM; and group ETS250 (n = 20) received standard chow supplemented with 250 mg/kg body weight/d of SM. The observation period was 2 months. The ETS animals had higher values of left cardiac chamber diameters and of left ventricular mass index. SM supplementation attenuated these changes. In addition, the myocyte cross-sectional area (CSA) was lower in group C compared with the ETS groups; however, the ETS250 group had lower values of CSA compared with the ETS group. The ETS group also showed higher cardiac levels of lipid hydroperoxide (LH) compared with group C; and, groups ETS100 and ETS250 had lower concentrations of LH compared with the ETS group. Regarding energy metabolism, SM supplementation decreased glycolysis and increased the β-oxidation and the oxidative phosphorylation. There were no differences in the expression of Nrf-2, SIRT-1, NF-κB, interferon-gamma and interleukin 10. In conclusion, our results suggest that ETS induced the cardiac remodelling process. In addition, SM supplementation attenuated this process, along with oxidative stress reduction and energy metabolism modulation.
本研究的目的是调查补充腰果树（SM）对大鼠暴露于烟草烟雾（ETS）所诱导的心脏重塑过程的影响。雄性Wistar大鼠分为4组：C组（对照组，n = 20）由未暴露于香烟烟雾且接受标准饲料的动物组成；ETS组（n = 20）由暴露于香烟烟雾且接受标准饲料的动物组成；ETS100组（n = 20）接受补充有100 mg/kg体重/天SM的标准饲料；ETS250组（n = 20）接受补充有250 mg/kg体重/天SM的标准饲料。观察期为2个月。ETS组动物的左心腔直径和左心室质量指数值更高。补充SM减弱了这些变化。此外，C组的心肌细胞横截面积（CSA）低于ETS组；然而，ETS250组的CSA值低于ETS组。与C组相比，ETS组的心脏脂质过氧化水平（LH）也更高；并且，与ETS组相比，ETS100组和ETS250组的LH浓度更低。关于能量代谢，补充SM降低了糖酵解并增加了β-氧化和氧化磷酸化。Nrf-2、SIRT-1、NF-κB、干扰素-γ和白细胞介素10的表达没有差异。总之，我们的结果表明ETS诱导了心脏重塑过程。此外，补充SM减弱了这一过程，同时降低了氧化应激并调节了能量代谢。
