Qureshi S, Saxena H M, Imam N, Kashoo Z, Sharief Banday M, Alam A, Malik Md Z, Ishrat R, Bhat B
Division of Veterinary Microbiology & Immunology, FVSc & A.H., Shuhama (Aulesteng), SKUAST-K, Shalimar, India.
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
Lett Appl Microbiol. 2018 Sep;67(3):244-253. doi: 10.1111/lam.13010. Epub 2018 Jul 17.
Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to differentiate between vaccinated and infected is essential. Pasteurella phage isolated in present study belongs to family Siphoviridae. PMP-GAD-IND phage exhibited lytic activity against vaccine strain (P52) as well as several field strains of P. multocida (B:2), and fowl cholera agent (P. multocida A:1).The phage has a double stranded DNA (dsDNA) with a genome of 46 335 bp. The complete genome sequence of the Pasteurella multocida phage has been deposited in Gen Bank with accession no: KY203335. PMP-GAD-IND being a lytic phage with broad activity range has a potential to be used in therapy against multidrug resistant P. multocida infections.
The present work is a part of research for the development of an improved phage lysate marker vaccine and a companion DIVA assay against haemorhagic septicaemia. This study describes the isolation and genome analysis of PMP-GAD-IND a lytic Pasteurella multocida bacteriophage.
目前用于预防多杀性巴氏杆菌B:2引起的出血性败血症的明矾沉淀疫苗和油佐剂疫苗有副作用且免疫期短，导致亚洲次大陆的牛群经常爆发灾难性疫情。因此，开发一种免疫期更长且能够区分接种动物和感染动物的改良疫苗至关重要。本研究分离出的多杀性巴氏杆菌噬菌体属于长尾噬菌体科。PMP-GAD-IND噬菌体对疫苗株（P52）以及多杀性巴氏杆菌的几种田间菌株（B:2）和禽霍乱病原体（多杀性巴氏杆菌A:1）表现出裂解活性。该噬菌体具有双链DNA（dsDNA），基因组大小为46335 bp。多杀性巴氏杆菌噬菌体的完整基因组序列已保存在Gen Bank中，登录号为：KY203335。PMP-GAD-IND作为一种具有广泛活性范围的裂解性噬菌体，有潜力用于治疗耐多药多杀性巴氏杆菌感染。
本工作是开发改良噬菌体裂解物标记疫苗和针对出血性败血症的配套鉴别诊断方法研究的一部分。本研究描述了裂解性多杀性巴氏杆菌噬菌体PMP-GAD-IND的分离和基因组分析。
