Shimizu K, Hirano T, Ishibashi K, Nakano N, Taga T, Sugamura K, Yamamura Y, Kishimoto T
J Immunol. 1985 Mar;134(3):1728-33.
Human peripheral T cells were transformed by human T cell leukemia virus (HTLV), and T cell lines producing BGDF (BCGF II) and BCDF were established. Among these cell lines, a cell line, TCL-Na1, secreted the highest level of both BGDF and BCDF, and the amount of BCDF secreted by TCL-Na1 cells was 900-fold more than that produced by PHA-stimulated T cells. Within the limits of our examination, none of the HTLV-transformed T cell lines produced IL 2 or BSF-p1 (BCGF I). BCDF produced by TCL-Na1 cells had a m.w. of 35,000 and a pI value of 5.5, being separated from BGDF, which was eluted in the fractions corresponding to m.w. of more than 60,000 and pI values of 5 to 6. BGDF induced both proliferation and IgM secretion in a mouse leukemic B cell line, BCL1, and these activities were not separated by either isoelectric focusing or gel filtration in the presence or absence of 0.1% Triton X-100, suggesting that the molecule designated BGDF exerted both growth and differentiation activities. BGDF acted on normal mouse B cells to induce proliferation as well as IgM secretion. The target cells of BGDF were in vivo activated B blast cells. BGDF acted on DXS-activated murine B cells to induce both proliferation and IgM secretion but not anti-Ig-activated B cells, indicating that BGDF and BSF-p1 were different molecules.
人外周血T细胞被人T细胞白血病病毒（HTLV）转化，建立了产生BGDF（BCGF II）和BCDF的T细胞系。在这些细胞系中，TCL-Na1细胞系分泌的BGDF和BCDF水平最高，TCL-Na1细胞分泌的BCDF量比PHA刺激的T细胞产生的量高900倍。在我们的检测范围内，没有一个HTLV转化的T细胞系产生IL-2或BSF-p1（BCGF I）。TCL-Na1细胞产生的BCDF分子量为35000，等电点为5.5，与BGDF分离，BGDF在分子量大于60000、等电点为5至6的级分中洗脱。BGDF在小鼠白血病B细胞系BCL1中诱导增殖和IgM分泌，在有或没有0.1% Triton X-100的情况下，这些活性通过等电聚焦或凝胶过滤都不能分开，这表明命名为BGDF的分子具有生长和分化活性。BGDF作用于正常小鼠B细胞以诱导增殖以及IgM分泌。BGDF的靶细胞是体内活化的B母细胞。BGDF作用于DXS活化的小鼠B细胞以诱导增殖和IgM分泌，但不作用于抗Ig活化的B细胞，这表明BGDF和BSF-p1是不同的分子。
