Fukushima A, Ueno H, Fujimoto S
Department of Immunology, Kochi Medical School, Nankoku, Japan.
Clin Exp Immunol. 1994 Mar;95(3):459-64. doi: 10.1111/j.1365-2249.1994.tb07019.x.
To investigate the relationship between uveitis and HTLV-I infection, we examined the cross-reactivity between HTLV-I antigens and retinal antigens recognized by T cells in B10.BR mice immunized with human HTLV-I-infected MT-2 cells. We found that T cells obtained from MT-2 immune mouse spleen responded not only to HTLV-I antigens but also to retinal antigens of various species. However, they did not respond to HTLV-I-negative lymphoid cell lines. Furthermore, established T cell lines from MT-2 immune spleen cells also responded to both HTLV-I and retinal antigens. The phenotype of the immune cells that responded to both HTLV-I and retinal antigens was CD4+, CD8-, and CD3+. The proliferative response of T cell lines to HTLV-I as well as various retinal antigens, was clearly blocked by addition of anti-CD3, anti-CD4, or anti-I-Ak MoAbs, but not by anti-CD8 antibody. The established T cell lines from HTLV-I immune spleen cells were all found to be CD3+, TCR beta +, CD4+, CD8- cells by flow cytometric analysis. These results indicate that an epitope of HTLV-I antigens is cross-reactive to an epitope of retinal antigens extracted from either human retinoblastoma or normal murine, rat, and bovine retinae at a T cell recognition level.
为了研究葡萄膜炎与人类嗜T淋巴细胞病毒I型（HTLV-I）感染之间的关系，我们检测了HTLV-I抗原与在用人HTLV-I感染的MT-2细胞免疫的B10.BR小鼠中T细胞识别的视网膜抗原之间的交叉反应性。我们发现，从MT-2免疫小鼠脾脏获得的T细胞不仅对HTLV-I抗原反应，而且对各种物种的视网膜抗原反应。然而，它们对HTLV-I阴性淋巴细胞系无反应。此外，从MT-2免疫脾细胞建立的T细胞系也对HTLV-I和视网膜抗原均有反应。对HTLV-I和视网膜抗原均有反应的免疫细胞表型为CD4+、CD8-和CD3+。T细胞系对HTLV-I以及各种视网膜抗原的增殖反应，通过添加抗CD3、抗CD4或抗I-Ak单克隆抗体可明显阻断，但抗CD8抗体则不能。通过流式细胞术分析发现，从HTLV-I免疫脾细胞建立的T细胞系均为CD3+、TCRβ+、CD4+、CD8-细胞。这些结果表明，在T细胞识别水平上，HTLV-I抗原的一个表位与从人类视网膜母细胞瘤或正常小鼠、大鼠和牛视网膜提取的视网膜抗原的一个表位具有交叉反应性。
