一种用于检测轮状病毒和腺病毒的联合酶免疫分析方法（EIARA）。

A combined enzyme immunoassay for rotavirus and adenovirus (EIARA).

作者信息

Pereira H G, Azeredo R S, Leite J P, Andrade Z P, De Castro L

出版信息

J Virol Methods. 1985 Jan;10(1):21-8. doi: 10.1016/0166-0934(85)90084-9.

Abstract

A combined enzyme immunoassay for rotavirus and adenovirus (EIARA) was developed as a double-antibody sandwich assay in which test samples are added to plastic wells coated with rotavirus or adenovirus goat antibody. The presence of antigens is detected by mixed-guinea pig antisera to the same viruses followed by rabbit anti-guinea pig IgG conjugated with peroxidase and subsequently by ortho-phenylenediamine substrate. Titrations of rotavirus (SA11) and adenovirus type 2 tissue culture grown viruses in the presence of separate capture sera and mixed detector sera revealed that the two virus-antibody reactions occurred independently and were not affected by each other. Comparison with another enzyme immunoassay for rotavirus, with immunoelectron microscopy and with polyacrylamide gel electrophoresis showed that EIARA is a sensitive and specific method for detecting rotaviruses and adenoviruses in faeces from children with gastroenteritis.

摘要

一种用于检测轮状病毒和腺病毒的联合酶免疫测定法（EIARA）被开发为一种双抗体夹心测定法，其中将测试样品添加到包被有轮状病毒或腺病毒山羊抗体的塑料孔中。通过与相同病毒的混合豚鼠抗血清检测抗原的存在，随后是与过氧化物酶缀合的兔抗豚鼠IgG，然后是邻苯二胺底物。在单独的捕获血清和混合检测血清存在的情况下，对轮状病毒（SA11）和2型腺病毒组织培养生长的病毒进行滴定，结果表明两种病毒 - 抗体反应独立发生且互不影响。与另一种用于轮状病毒的酶免疫测定法、免疫电子显微镜和聚丙烯酰胺凝胶电泳进行比较，结果表明EIARA是一种检测胃肠炎患儿粪便中轮状病毒和腺病毒的灵敏且特异的方法。