Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
Molecules. 2018 May 28;23(6):1290. doi: 10.3390/molecules23061290.
In order to investigate the effect of benzothiadiazole (BTH) and β-aminobutyric acid (BABA) on the resistance of tea plants ) to tea geometrid (), three levels each of benzothiadiazole (BTH) and β-aminobutyric acid (BABA) were sprayed on 10-year-old tea plants. Generally PPO and PAL activities increased with low concentrations of BTH and BABA treatments. Quantitative RT-PCR revealed a 1.43 and 2.72-fold increase in PPO gene expression, and 3.26 and 3.99-fold increase in PAL gene expression with 75 mg/L BTH and 400 mg/L BABA respectively. Analysis of hydrolysis of synthetic substrates also revealed that chymotrypsin-like enzyme activity present in larval midgut extracts was not significantly inhibited by BTH and BABA. However, proteinase activity was found to be inversely proportional to the age of tea geometrid. Larvae pupation rate decreased by 8.10, 10.81 and 21.62% when tea geometrid were fed with leaves treated with 25, 50 and 75 mg/L BTH solutions, while 100, 200 and 400 mg/L BABA solutions decreased same by 8.10, 16.21 and 13.51% respectively. Also, larvae development period delayed to 23.33 and 26.33 days with 75 mg/L BTH and 400 mg/L BABA treatments respectively. The results in this study; therefore, suggest that benzothiadiazole (BTH) and β-aminobutyric acid (BABA) play a role in inducing resistance in tea plants to tea geometrid, with the optimal effect achieved at BTH-3 (75 mg/L) and BABA-3 (400 mg/L), respectively.
为了研究噻苯隆（BTH）和β-氨基丁酸（BABA）对茶树（）对茶尺蠖抗性的影响，在 10 年生茶树上喷施了 3 个浓度的噻苯隆（BTH）和β-氨基丁酸（BABA）。一般来说，PPO 和 PAL 活性随着 BTH 和 BABA 处理浓度的降低而增加。定量 RT-PCR 显示，PPO 基因表达分别增加了 1.43 倍和 2.72 倍，PAL 基因表达分别增加了 3.26 倍和 3.99 倍，用 75mg/L BTH 和 400mg/L BABA 处理。水解合成底物的分析也表明，幼虫中肠提取物中的胰凝乳蛋白酶样酶活性没有被 BTH 和 BABA 显著抑制。然而，蛋白酶活性与茶尺蠖的年龄成反比。当茶尺蠖取食用 25、50 和 75mg/L BTH 溶液处理的叶片时，化蛹率分别下降了 8.10%、10.81%和 21.62%，而 100、200 和 400mg/L BABA 溶液处理时，化蛹率分别下降了 8.10%、16.21%和 13.51%。此外，用 75mg/L BTH 和 400mg/L BABA 处理时，幼虫发育期分别延迟到 23.33 和 26.33 天。因此，本研究结果表明，噻苯隆（BTH）和β-氨基丁酸（BABA）在诱导茶树对茶尺蠖的抗性方面发挥作用，BTH-3（75mg/L）和 BABA-3（400mg/L）的效果最佳。
