Swann K, Whitaker M
Nature. 1985;314(6008):274-7. doi: 10.1038/314274a0.
On fertilization of a sea urchin egg, marked changes occur in the cytoplasmic concentration of calcium and hydrogen ions. These ionic signals represent the necessary and sufficient stimuli for the increased metabolism, protein synthesis and DNA synthesis that constitute egg activation. Cytoplasmic alkalinization, the major immediate cause of the increased rate of protein synthesis which occurs at fertilization, arises because the sperm-induced intracellular calcium transient activates a coupled flux of sodium ions and hydrogen ions across the oolemma. The experiments reported here suggest that the second messenger which links the activation of the Na/H exchange to the calcium transient may be a substance which stimulates protein kinase C8, as 12-O-tetradecanoyl phorbol acetate (TPA), a known activator of protein kinase C9, appears to stimulate protein synthesis by turning on the Na/H exchanger and causing a cytoplasmic alkalinization. Our data indicate that one consequence of treating other tissues with TPA, a tumour promoter, may be an increase in intracellular pH.
海胆卵受精时，钙离子和氢离子的细胞质浓度会发生显著变化。这些离子信号是构成卵激活的新陈代谢增加、蛋白质合成和DNA合成的必要且充分刺激因素。细胞质碱化是受精时蛋白质合成速率增加的主要直接原因，这是因为精子诱导的细胞内钙瞬变激活了钠离子和氢离子跨卵膜的耦合通量。本文报道的实验表明，将钠/氢交换的激活与钙瞬变联系起来的第二信使可能是一种刺激蛋白激酶C8的物质，因为已知的蛋白激酶C9激活剂12-O-十四酰佛波醇乙酸酯（TPA）似乎通过开启钠/氢交换器并导致细胞质碱化来刺激蛋白质合成。我们的数据表明，用肿瘤促进剂TPA处理其他组织的一个后果可能是细胞内pH值升高。
