Kuhnt U, Mihály A, Joó F
Neurosci Lett. 1985 Jan 21;53(2):149-54. doi: 10.1016/0304-3940(85)90177-6.
Long-term potentiation (LTP) of CA1 pyramidal neurons was induced by tetanic stimulation in the stratum radiatum of hippocampal slices from guinea pigs. Unstimulated and stimulated slices were treated using a histochemical procedure enabling the electron microscopic (EM) visualization of Ca binding sites. Electron-dense, Ca-containing deposits were found in low numbers in unstimulated slices on pre- and postsynaptic sites. In the stratum radiatum of tetanized slices the overall number of deposits as well as the number of deposits in dendrites was clearly increased. The results support the hypothesis that Ca-dependent postsynaptic mechanisms are important for the generation of LTP.
通过对豚鼠海马切片辐射层进行强直刺激，诱导CA1锥体神经元的长时程增强（LTP）。使用一种组织化学方法处理未刺激和刺激的切片，该方法能够在电子显微镜（EM）下观察钙结合位点。在未刺激切片的突触前和突触后位点发现少量电子致密的含钙沉积物。在接受强直刺激的切片辐射层中，沉积物的总数以及树突中的沉积物数量明显增加。这些结果支持了以下假设，即钙依赖的突触后机制对LTP的产生很重要。
