Groenen M A, Timmers E, van de Putte P
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2087-91. doi: 10.1073/pnas.82.7.2087.
We have determined the minimal DNA sequences at the ends of the genome of bacteriophage Mu that are required for its transposition. A mini-Mu was constructed on a multicopy plasmid that enabled the manipulation of the DNA sequences at its ends without affecting the genes essential for transposition. The genes A and B, which were cloned outside the ends of the mini-Mu on the same plasmid, were both needed for optimal transposition. In our experimental system the predominant end products of the transposition are cointegrates both in the presence and in the absence of B. Two regions ending approximately 25 and 160 bp from the left end and one ending approximately 50 bp from the right end appear to be essential for optimal transposition. Overlapping with these regions, a 22-base-pair sequence was recognized with the consensus Y-G-T-T-C-A-Y-T-N-N-A-A-R-Y-R-C-G-A-A-A-A, where Y and R represent any pyrimidine and purine, respectively. At the left end these sequences occur as direct repeats; at the right end this sequence is inverted with respect to those at the left end.
我们已经确定了噬菌体Mu基因组末端对于其转座所必需的最小DNA序列。在多拷贝质粒上构建了一个微型Mu，这使得能够对其末端的DNA序列进行操作，而不影响转座所必需的基因。克隆在微型Mu末端之外同一质粒上的A和B基因，对于最佳转座都是必需的。在我们的实验系统中，无论有无B，转座的主要终产物都是共整合体。从左端起大约25和160 bp处结束的两个区域以及从右端起大约50 bp处结束的一个区域，对于最佳转座似乎至关重要。与这些区域重叠的是一个22个碱基对的序列，其共有序列为Y-G-T-T-C-A-Y-T-N-N-A-A-R-Y-R-C-G-A-A-A-A，其中Y和R分别代表任何嘧啶和嘌呤。在左端，这些序列以正向重复出现；在右端，该序列相对于左端的序列是反向的。
