Harel J, DuBow M S
Department of Microbiology and Immunology, McGill University, Montr al, Québec, Canada.
Arch Microbiol. 1994;161(5):418-24. doi: 10.1007/BF00288953.
Bacteriophage Mu DNA, like other transposable elements, requires DNA sequences at both extremities to transpose. It has been previously demonstrated that the transposition activity of various transposons can be influenced by sequences outside their ends. We have found that alterations in the neighboring plasmid sequences near the right extremity of a Mini-Mu, inserted in the plasmid pSC101, can exert an influence on the efficiency of Mini-Mu DNA transposition when an induced helper Mu prophage contains a polar insertion in its semi-essential early region (SEER). The SEER of Mu is known to contain several genes that can affect DNA transposition, and our results suggest that some function(s), located in the SEER of Mu, may be required for optimizing transposition (and thus, replication) of Mu genomes from restrictive locations during the lytic cycle.
噬菌体Mu DNA与其他转座元件一样，转座时需要两端的DNA序列。此前已经证明，各种转座子的转座活性会受到其末端以外序列的影响。我们发现，插入质粒pSC101中的Mini-Mu右端附近的相邻质粒序列发生改变时，当诱导的辅助噬菌体Mu原噬菌体在其半必需早期区域（SEER）含有极性插入时，会对Mini-Mu DNA的转座效率产生影响。已知Mu的SEER包含几个会影响DNA转座的基因，我们的结果表明，Mu的SEER中存在的某些功能可能是在裂解周期中从限制性位点优化Mu基因组的转座（进而复制）所必需的。
