Kazan Federal University, Kazan 420008, Russia.
Biomed Res Int. 2018 Apr 8;2018:7053623. doi: 10.1155/2018/7053623. eCollection 2018.
Extracellular vesicles (EV) represent a promising vector system for biomolecules and drug delivery due to their natural origin and participation in intercellular communication. As the quantity of EVs is limited, it was proposed to induce the release of membrane vesicles from the surface of human cells by treatment with cytochalasin B. Cytochalasin B-induced membrane vesicles (CIMVs) were successfully tested as a vector for delivery of dye, nanoparticles, and a chemotherapeutic. However, it remained unclear whether CIMVs possess fusion specificity with target cells and thus might be used for more targeted delivery of therapeutics. To answer this question, CIMVs were obtained from human prostate cancer PC3 cells. The diameter of obtained CIMVs was 962,13 ± 140,6 nm. We found that there is no statistically significant preference in PC3 CIMVs fusion with target cells of the same type. According to our observations, the greatest impact on CIMVs entry into target cells is by the heterophilic interaction of CIMV membrane receptors with the surface proteins of target cells.
细胞外囊泡 (EV) 因其天然来源和参与细胞间通讯而成为生物分子和药物传递的有前途的载体系统。由于 EV 的数量有限，有人提出通过用细胞松弛素 B 处理来诱导人细胞表面释放膜囊泡。细胞松弛素 B 诱导的膜囊泡 (CIMVs) 已成功测试作为染料、纳米颗粒和化疗药物的传递载体。然而，CIMVs 是否与靶细胞具有融合特异性，因此是否可用于更靶向的治疗药物传递仍然不清楚。为了回答这个问题，从人前列腺癌细胞 PC3 中获得了 CIMVs。获得的 CIMVs 的直径为 962.13 ± 140.6nm。我们发现，PC3 CIMVs 与同种类型的靶细胞融合没有统计学上的偏好。根据我们的观察，对 CIMVs 进入靶细胞的最大影响是 CIMV 膜受体与靶细胞表面蛋白的异嗜性相互作用。
