Gilazieva Zarema, Chulpanova Daria, Ponomarev Aleksei, Filin Ivan, Garanina Ekaterina, Rizvanov Albert, Solovyeva Valeriya
Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Curr Issues Mol Biol. 2022 Nov 1;44(11):5363-5378. doi: 10.3390/cimb44110363.
To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which affect similar types of cells in the opposite way. Therefore, it is important to study and compare methods of vesicle isolation. Moreover, the differences between the EVs derived from tumor and mesenchymal stem cells are still poorly understood. This article compares EVs from human glioblastoma cells and mesenchymal stem cells (MSCs) obtained by two different methods, ultracentrifugation and cytochalasin B-mediated induction. The size of the vesicles, the presence of the main EV markers, the presence of nuclear and mitochondrial components, and the molecular composition of the vesicles were determined. It has been shown that EVs obtained by both ultracentrifugation and cytochalasin B treatment have similar features, contain particles of endogenous and membrane origin and can interact with monolayer cultures of tumor cells.
迄今为止，有众多用于分离细胞外囊泡（EVs）的方案。根据分离方法的不同，有可能获得具有不同特性的囊泡，这些囊泡富含特定的蛋白质、DNA和RNA组群，它们以相反的方式影响相似类型的细胞。因此，研究和比较囊泡分离方法很重要。此外，源自肿瘤细胞和间充质干细胞的细胞外囊泡之间的差异仍知之甚少。本文比较了通过两种不同方法（超速离心和细胞松弛素B介导的诱导）从人胶质母细胞瘤细胞和间充质干细胞（MSCs）获得的细胞外囊泡。测定了囊泡的大小、主要细胞外囊泡标志物的存在情况、核和线粒体成分的存在情况以及囊泡的分子组成。结果表明，通过超速离心和细胞松弛素B处理获得的细胞外囊泡具有相似的特征，包含内源性和膜源性颗粒，并且可以与肿瘤细胞的单层培养物相互作用。
